TAF5 Antibody

Code CSB-PA613596ESR2HU
Size US$166
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Image
  • Western blot
    All lanes: TAF5 antibody at 1.74 μg/ml
    Lane 1: Jurkat whole cell lysate
    Lane 2: MCF-7 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 87, 81 kDa
    Observed band size: 87, 40, 51 kDa

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA613596ESR2HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) TAF5 Polyclonal antibody
Uniprot No.
Target Names
TAF5
Alternative Names
TAF5 antibody; TAF2D antibody; Transcription initiation factor TFIID subunit 5 antibody; Transcription initiation factor TFIID 100 kDa subunit antibody; TAF(II)100 antibody; TAFII-100 antibody; TAFII100 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Transcription initiation factor TFIID subunit 5 protein (656-800AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed.
Gene References into Functions
  1. TFIID TAF6-TAF9 complex formation involves the HEAT repeat-containing C-terminal domain of TAF6 and is modulated by TAF5 protein. PMID: 22696218
  2. reversible SUMO modification at hsTAF5 contributes to the dynamic regulation of TFIID promoter-binding activity in human cells PMID: 15637059
  3. TAF5 protein demonstrates the ability of the N-terminal half of the TAF5 gene to form a flexible, extended dimer, a key property required for the assembly of the TFIID complex PMID: 17227857
Subcellular Location
Nucleus.
Protein Families
WD repeat TAF5 family
Database Links

HGNC: 11539

OMIM: 601787

KEGG: hsa:6877

STRING: 9606.ENSP00000358854

UniGene: Hs.96103

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