Name: TGS1 Antibody
Brand: CUSABIO
SKU: CSB-PA847219LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TGS1 Polyclonal antibody

Uniprot No.

Q96RS0

Target Names

TGS1

Alternative Names

Cap specific guanine N2 methyltransferase antibody; Cap-specific guanine-N2 methyltransferase antibody; CLL associated antigen KW 2 antibody; CLL-associated antigen KW-2 antibody; DKFZp762A163 antibody; FLJ22995 antibody; HCA137 antibody; Hepatocellular carcinoma associated antigen 137 antibody; Hepatocellular carcinoma-associated antigen 137 antibody; NCOA6IP antibody; Nuclear receptor coactivator 6 interacting protein antibody; Nuclear receptor coactivator 6-interacting protein antibody; PIMT antibody; PIPMT antibody; PRIP interacting protein PIPMT antibody; PRIP interacting protein with methyltransferase domain antibody; PRIP interacting protein with methyltransferase motif antibody; PRIP-interacting protein with methyltransferase motif antibody; SEREX defined antibody; TGS 1 antibody; Tgs1 antibody; TGS1_HUMAN antibody; Trimethylguanosine synthase antibody; Trimethylguanosine synthase homolog (S. cerevisiae) antibody; Trimethylguanosine synthase homolog antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Trimethylguanosine synthase protein (713-853AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TGS1 Antibody (Product code: CSB-PA847219LA01HU) is Non-conjugated. For TGS1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA847219LB01HU	TGS1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA847219LC01HU	TGS1 Antibody, FITC conjugated
Biotin	CSB-PA847219LD01HU	TGS1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.

Gene References into Functions

Data highlight the importance of the catalytic activity of PIMT to mediate VEGF effects during endothelial cell migration and tube formation in angiogenesis. PMID: 26738492
Self-association of Tgs1p and its catalytic activity were prerequisite to bypass the requirement for its accessory factor Swm2p for efficient pre-rRNA processing and snRNA trimethylation. PMID: 26074133
PIMT was identified as a key player responsible for glycated low density lipoproteins induced vascular endothelial cell apoptosis. PMID: 23922881
isoforms of the PIMT/Tgs1 protein with an RNA methyltransferase domain function both in the nucleus and in the cytoplasm PMID: 12943661
The protein L-isoaspartyl (D-aspartyl) methyltransferase (PIMT) is an enzyme that recognizes and repairs the abnormal L-isoaspartyl residues in proteins. PMID: 21204776
The structure-function data highlight a strictly essential pi-cation interaction between Trp766 and the m(7)G base and a network of important enzymic contacts to the cap triphosphate via Lys646, Tyr771, Arg807, and Lys836. PMID: 20360394
Interaction of PIMT with transcriptional coactivators CBP, p300, and PBP differential role in transcriptional regulation. PMID: 11912212
proteasome maturation constitutes a mechanism regulating Tgs1 function by generating Tgs1 species with different substrate specificities, subcellular localizations, and functions. PMID: 18039666
These results highlight that PIMT expression is regulated by ROS and could primarily act as an antioxidant enzyme. PMID: 18407833
present a biochemical characterization of the human Tgs1 guanine-N2 methyltransferase reaction and identify individual amino acids required for methyltransferase activity in vitro and in vivo PMID: 18775984
m(7)GpppA binds via its adenosine moiety to the structurally conserved adenosylmethionine-binding pocket. The m(7) guanosine is unbound. The crystallized TGS1 fragment is catalytically inactive, but a fragment that is 17 AAs longer exhibits activity. PMID: 19307714
The crystal structure of the substrate bound methyltransferase domain as well as mutagenesis studies provide insight into the catalytic mechanism of TGS1. PMID: 19386620

Hide All

Subcellular Location

Cytoplasm. Nucleus, Cajal body. Nucleus, nucleolus.

Protein Families

Methyltransferase superfamily, Trimethylguanosine synthase family

Tissue Specificity

Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta.

Database Links

HGNC: 17843

OMIM: 606461

KEGG: hsa:96764

STRING: 9606.ENSP00000260129

UniGene: Hs.335068

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Code	CSB-PA847219LA01HU
Size	US$166
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Image	Western blot All lanes: TGS1 antibody at 8µg/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 97 kDa Observed band size: 85 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA847219LA01HU at dilution of 1:100
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TGS1 Antibody

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Target Background