TGS1 Antibody

Code CSB-PA847219LA01HU
Size US$166
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  • Western blot
    All lanes: TGS1 antibody at 8µg/ml + Mouse heart tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 97 kDa
    Observed band size: 85 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA847219LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) TGS1 Polyclonal antibody
Uniprot No.
Target Names
TGS1
Alternative Names
Cap specific guanine N2 methyltransferase antibody; Cap-specific guanine-N2 methyltransferase antibody; CLL associated antigen KW 2 antibody; CLL-associated antigen KW-2 antibody; DKFZp762A163 antibody; FLJ22995 antibody; HCA137 antibody; Hepatocellular carcinoma associated antigen 137 antibody; Hepatocellular carcinoma-associated antigen 137 antibody; NCOA6IP antibody; Nuclear receptor coactivator 6 interacting protein antibody; Nuclear receptor coactivator 6-interacting protein antibody; PIMT antibody; PIPMT antibody; PRIP interacting protein PIPMT antibody; PRIP interacting protein with methyltransferase domain antibody; PRIP interacting protein with methyltransferase motif antibody; PRIP-interacting protein with methyltransferase motif antibody; SEREX defined antibody; TGS 1 antibody; Tgs1 antibody; TGS1_HUMAN antibody; Trimethylguanosine synthase antibody; Trimethylguanosine synthase homolog (S. cerevisiae) antibody; Trimethylguanosine synthase homolog antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Trimethylguanosine synthase protein (713-853AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The TGS1 Antibody (Product code: CSB-PA847219LA01HU) is Non-conjugated. For TGS1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA847219LB01HU TGS1 Antibody, HRP conjugated ELISA
FITC CSB-PA847219LC01HU TGS1 Antibody, FITC conjugated
Biotin CSB-PA847219LD01HU TGS1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.
Gene References into Functions
  1. Data highlight the importance of the catalytic activity of PIMT to mediate VEGF effects during endothelial cell migration and tube formation in angiogenesis. PMID: 26738492
  2. Self-association of Tgs1p and its catalytic activity were prerequisite to bypass the requirement for its accessory factor Swm2p for efficient pre-rRNA processing and snRNA trimethylation. PMID: 26074133
  3. PIMT was identified as a key player responsible for glycated low density lipoproteins induced vascular endothelial cell apoptosis. PMID: 23922881
  4. isoforms of the PIMT/Tgs1 protein with an RNA methyltransferase domain function both in the nucleus and in the cytoplasm PMID: 12943661
  5. The protein L-isoaspartyl (D-aspartyl) methyltransferase (PIMT) is an enzyme that recognizes and repairs the abnormal L-isoaspartyl residues in proteins. PMID: 21204776
  6. The structure-function data highlight a strictly essential pi-cation interaction between Trp766 and the m(7)G base and a network of important enzymic contacts to the cap triphosphate via Lys646, Tyr771, Arg807, and Lys836. PMID: 20360394
  7. Interaction of PIMT with transcriptional coactivators CBP, p300, and PBP differential role in transcriptional regulation. PMID: 11912212
  8. proteasome maturation constitutes a mechanism regulating Tgs1 function by generating Tgs1 species with different substrate specificities, subcellular localizations, and functions. PMID: 18039666
  9. These results highlight that PIMT expression is regulated by ROS and could primarily act as an antioxidant enzyme. PMID: 18407833
  10. present a biochemical characterization of the human Tgs1 guanine-N2 methyltransferase reaction and identify individual amino acids required for methyltransferase activity in vitro and in vivo PMID: 18775984
  11. m(7)GpppA binds via its adenosine moiety to the structurally conserved adenosylmethionine-binding pocket. The m(7) guanosine is unbound. The crystallized TGS1 fragment is catalytically inactive, but a fragment that is 17 AAs longer exhibits activity. PMID: 19307714
  12. The crystal structure of the substrate bound methyltransferase domain as well as mutagenesis studies provide insight into the catalytic mechanism of TGS1. PMID: 19386620

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Subcellular Location
Cytoplasm. Nucleus, Cajal body. Nucleus, nucleolus.
Protein Families
Methyltransferase superfamily, Trimethylguanosine synthase family
Tissue Specificity
Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta.
Database Links

HGNC: 17843

OMIM: 606461

KEGG: hsa:96764

STRING: 9606.ENSP00000260129

UniGene: Hs.335068

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