TJP2 Antibody

Datasheet
Code CSB-PA880071NA01HU
Size US$299
Uniprot No. Q9UDY2
Image
  • IHC image of CSB-PA880071NA01HU diluted at 1:600 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of U251 cells with CSB-PA880071NA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate
    All lanes: TJP2 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 134, 118, 112, 132, 116, 131, 138 kDa
    Observed band size: 150 kDa

Immunogen Recombinant Human Tight junction protein ZO-2 protein (1165-1183AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:200-1:500
Relevance Plays a role in tight junctions and adherens junctions.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Tight junction protein ZO-2 (Tight junction protein 2) (Zona occludens protein 2) (Zonula occludens protein 2), TJP2, X104 ZO2
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Target Names TJP2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions? Leave a Message or Start an on-line Chat
Function Plays a role in tight junctions and adherens junctions.
Involvement in disease Familial hypercholanemia (FHCA); Cholestasis, progressive familial intrahepatic, 4 (PFIC4)
Subcellular Location Cell junction, adherens junction, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cell junction, tight junction, Nucleus
Protein Families MAGUK family
Tissue Specificity This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, he
Database Links

HGNC: 11828

OMIM: 607709

KEGG: hsa:9414

STRING: 9606.ENSP00000438262

UniGene: Hs.50382

Related Products

TJP2 Antibodies

TJP2 Antibodies for Homo sapiens (Human)

TJP2 Proteins

TJP2 Proteins for Homo sapiens (Human)

TJP2 ELISA Kit

TJP2 ELISA Kit for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.