TMF1 Antibody

Datasheet

Code	CSB-PA023904LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All lanes: TMF1 antibody at 3.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 124 kDa Observed band size: 123, 124 kDa IHC image of CSB-PA023904LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TMF1 Polyclonal antibody

Uniprot No.

P82094

Target Names

TMF1

Alternative Names

Androgen receptor coactivator 160 kDa protein antibody; Androgen receptor-associated protein of 160 kDa antibody; ARA160 antibody; TATA element modulatory factor 1 antibody; TATA element modulatory factor antibody; TMF antibody; Tmf1 antibody; TMF1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human TATA element modulatory factor protein (878-986AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TMF1 Antibody (Product code: CSB-PA023904LA01HU) is Non-conjugated. For TMF1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA023904LB01HU	TMF1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA023904LC01HU	TMF1 Antibody, FITC conjugated
Biotin	CSB-PA023904LD01HU	TMF1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP).

Gene References into Functions

In patient with chronic granulomatous disease, a 5.8-kb, incomplete and partly exonized TMF1 transcript was identified in intron 1 of CYBB, in opposite orientation to the host gene. PMID: 24478191
COG complex interacts with two different Rabs in addition to each end of the golgin "TATA element modulatory factor" (TMF). PMID: 23239882
These observations suggest critical roles for TMF in two Rab6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the endoplasmic reticulum. PMID: 17698061
Overexpression of TMF/ARA160 downregulates proangiogenic genes and attenuates the progression of prostate cancer xenografts. PMID: 19330832

Subcellular Location

Cytoplasm. Nucleus. Golgi apparatus membrane. Note=Concentrated at the budding structures localized at the tips of cisternae.

Database Links

HGNC: 11870

OMIM: 601126

KEGG: hsa:7110

STRING: 9606.ENSP00000381567

UniGene: Hs.267632

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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