TMF1 Antibody

Code CSB-PA023904LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
    All lanes: TMF1 antibody at 3.7µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 123, 124 kDa
    Observed band size: 123, 124 kDa

  • IHC image of CSB-PA023904LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) TMF1 Polyclonal antibody
Uniprot No.
Target Names
TMF1
Alternative Names
Androgen receptor coactivator 160 kDa protein antibody; Androgen receptor-associated protein of 160 kDa antibody; ARA160 antibody; TATA element modulatory factor 1 antibody; TATA element modulatory factor antibody; TMF antibody; Tmf1 antibody; TMF1_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human TATA element modulatory factor protein (878-986AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The TMF1 Antibody (Product code: CSB-PA023904LA01HU) is Non-conjugated. For TMF1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA023904LB01HU TMF1 Antibody, HRP conjugated ELISA
FITC CSB-PA023904LC01HU TMF1 Antibody, FITC conjugated
Biotin CSB-PA023904LD01HU TMF1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP).
Gene References into Functions
  1. In patient with chronic granulomatous disease, a 5.8-kb, incomplete and partly exonized TMF1 transcript was identified in intron 1 of CYBB, in opposite orientation to the host gene. PMID: 24478191
  2. COG complex interacts with two different Rabs in addition to each end of the golgin "TATA element modulatory factor" (TMF). PMID: 23239882
  3. These observations suggest critical roles for TMF in two Rab6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the endoplasmic reticulum. PMID: 17698061
  4. Overexpression of TMF/ARA160 downregulates proangiogenic genes and attenuates the progression of prostate cancer xenografts. PMID: 19330832
Subcellular Location
Cytoplasm. Nucleus. Golgi apparatus membrane. Note=Concentrated at the budding structures localized at the tips of cisternae.
Database Links

HGNC: 11870

OMIM: 601126

KEGG: hsa:7110

STRING: 9606.ENSP00000381567

UniGene: Hs.267632

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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