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TOMM20 Antibody

Datasheet
Code CSB-PA618983ESR2HU
Size US$166
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Image
  • Western Blotting(WB) - TOMM20 Antibody

    Western blot
    All lanes: Mitochondrial import receptor subunit TOM20 homolog antibody at 3μg/ml
    Lane 1: Mouse brain tissue
    Lane 2: Hela whole cell lysate
    Lane 3: Mouse liver tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Immunohistochemistry(IHC) 1- TOMM20 Antibody

    Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA618983ESR2HU at dilution of 1:100

  • Immunofluorescence(IF) - TOMM20 Antibody

    Immunofluorescent analysis of HepG2 cells using CSB-PA618983ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) TOMM20 Polyclonal antibody
Uniprot No.
Q15388
Target Names
TOMM20
Alternative Names
KIAA0016 antibody; MAS20 antibody; MGC117367 antibody; Mitochondrial 20 kDa outer membrane protein antibody; Mitochondrial import receptor subunit TOM20 homolog antibody; MOM19 antibody; Outer mitochondrial membrane receptor Tom20 antibody; TOM20 antibody; TOM20_HUMAN antibody; TOMM20 antibody; Translocase of outer mitochondrial membrane 20 homolog (yeast) antibody; Translocase of outer mitochondrial membrane 20 homolog type II antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Mitochondrial import receptor subunit TOM20 homolog protein (20-145AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Related Products

TOMM20 antibodies

TOMM20 Proteins

Customer Reviews and Q&A

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Target Background

Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Gene References into Functions
  1. TOMM20 and MCT1 were highly expressed in diffuse large B-cell lymphoma lymphocytes, indicating an OXPHOS phenotype, whereas non-neoplastic lymphocytes in the control samples did not express these markers. PMID: 29248132
  2. TOMM20, MCT1, and MCT4 expression was significantly different in Hodgkin and Reed Sternberg (HRS) cells. HRS have high expression of TOMM20, while tumor associated macrophages have absent TOMM20 expression. Tumor-infiltrating lymphocytes have low TOMM20 expression. Reactive lymph nodes in contrast to cHL tumors had low TOMM20, MCT1, and MCT4 expression in lymphocytes and macrophages. PMID: 29248133
  3. Gsk3beta and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease PMID: 27503909
  4. TOMM20 protein is highly expressed in both anaplastic thyroid cancer and papillary thyroid cancer compared to non-cancerous thyroid tissue. PMID: 26615136
  5. Tom20 was ubiquitously distributed in the organ of Corti, allowing well-delineated visualization of inner and outer hair cells. In the inner ear of aged subjects with Meniere's disease, there was decreased expression of Tom20. PMID: 23165776
  6. a novel model in which Tom20 binds Tom70 to facilitate preprotein release from the chaperones by competition PMID: 21771790
  7. Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins PMID: 11913975
  8. the cytosolic domains of Tom20 and Tom22 function to maintain their substrate preproteins unfolded and prevent them from aggregating on the mitochondrial surface PMID: 14699115

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Subcellular Location
Mitochondrion outer membrane; Single-pass membrane protein.
Protein Families
Tom20 family
Database Links

HGNC: 20947

OMIM: 601848

KEGG: hsa:9804

STRING: 9606.ENSP00000355566

UniGene: Hs.533192

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