TPST1 Antibody

Datasheet

Code	CSB-PA024132LA01HU
Size	$600
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Image	IHC image of CSB-PA024132LA01HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA024132LA01HU diluted at 1:50 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Western Blot Positive WB detected in: Mouse Skeletal Muscle tissue lysate(20µg),Rat Skeletal Muscle tissue lysate(20µg) All lanes: TPST1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 120s Western Blot Positive WB detected in: A549 whole cell lysate(20µg), U251 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), Hela whole cell lysate(20µg), PC-3 whole cell lysate(20µg), 293 whole cell lysate(20µg) All lanes: TPST1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 60s
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TPST1 Polyclonal antibody

Uniprot No.

O60507

Target Names

TPST1

Alternative Names

TPST1; Protein-tyrosine sulfotransferase 1; Tyrosylprotein sulfotransferase 1; TPST-1

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Protein-tyrosine sulfotransferase 1 protein (283-370AA)

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.02% sodium azide
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:3000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.

Gene References into Functions

TPST1 was significantly negatively correlated with the expression of cMet in lung cancer and may be a negative prognostic biomarker of lung cancer. PMID: 26238632
TPST1 rs3757417T>G polymorphisms are associated with colorectal cancer. PMID: 25079514
Affinity purified salivary TPST showed a single band of 50-54 kDa and is the first report characterizing a tyrosylprotein sulfotransferase in secretory fluid from the parotid gland PMID: 16244708
results exclude TPST1 as the causative gene for Shwachman-Diamond syndrome PMID: 12032733
Shear stress-dependent downregulation of TPST1 in human endothelium involves protein kinase C PMID: 12056800
Human tyrosylprotein sulfotransferase may be functional as homodimer/oligomer in the trans-Golgi compartment. PMID: 16859706

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Subcellular Location

Golgi apparatus membrane; Single-pass type II membrane protein.

Protein Families

Protein sulfotransferase family

Tissue Specificity

Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Database Links

HGNC: 12020

OMIM: 603125

KEGG: hsa:8460

STRING: 9606.ENSP00000302413

UniGene: Hs.421194

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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