TUBA4A Antibody

Datasheet

Code	CSB-PA09499A0Rb
Size	US$166
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Image	Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse brain tissue All lanes: TUBA4A antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 49 kDa Observed band size: 50, 24 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA09499A0Rb at dilution of 1:100 Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA09499A0Rb at dilution of 1:100 Immunofluorescent analysis of HepG2 cells using CSB-PA09499A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Immunoprecipitating TUBA4A in K562 whole cell lysate Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA09499A0Rb in K562 whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: CSB-PA09499A0Rb (4μg) + K562 whole cell lysate (500μg) Lane 3: K562 whole cell lysate (20μg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TUBA4A Polyclonal antibody

Uniprot No.

P68366

Target Names

TUBA4A

Alternative Names

Alpha-tubulin 1 antibody; ALS22 antibody; B ALPHA 1 antibody; bA408E5.3 antibody; H2 ALPHA antibody; Hum a tub1 antibody; Hum a tub2 antibody; LIS3 antibody; MGC171407 antibody; MGC55332 antibody; TBA4A_HUMAN antibody; Testis-specific alpha-tubulin antibody; TUBA1 antibody; TUBA1A antibody; tuba1l antibody; Tuba4a antibody; Tubulin alpha 1 chain antibody; Tubulin alpha antibody; Tubulin alpha-1 chain antibody; tubulin alpha-1B chain antibody; Tubulin alpha-4A chain antibody; Tubulin H2-alpha antibody; Tubulin, alpha 1 (testis specific) antibody; tubulin, alpha 1, like antibody; Tubulin, alpha 4a antibody; Tubulin, alpha, testis-specific antibody; Tubulin, alpha-1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Tubulin alpha-4A chain protein (20-183AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TUBA4A Antibody (Product code: CSB-PA09499A0Rb) is Non-conjugated. For TUBA4A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA09499B0Rb	TUBA4A Antibody, HRP conjugated	ELISA
FITC	CSB-PA09499C0Rb	TUBA4A Antibody, FITC conjugated
Biotin	CSB-PA09499D0Rb	TUBA4A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Gene References into Functions

Data indicate a microRNA-1825/TBCB/TUBA4A pathway as a putative pathogenic cascade in both familial ALS (fALS) and both sporadic (sALS). PMID: 30030593
The elevated selenium species levels in the TUBA4A patient may have a genetic etiology and/or represent a pathogenic pathway through which this mutation favors disease onset. PMID: 28478440
alphaTubulin expression might be an independent prognostic factor for pancreatic cancer after surgical resection PMID: 27447976
Its mutations were seen in patients with ALS and frontotemporal dementia. PMID: 28069311
TUBA4A mutations are not associated with FTD. PMID: 26675813
In conclusion, our current results did not find an association between TUBA4A and ALS in Chinese patients, and further studies will be required. PMID: 26465396
This study mutational screening revealed the presence of four novel heterozygous variants of TUBA4A mutation in four sporadic amyotrophic lateral sclerosis. PMID: 25893256
Data show that overexpression of the 14-3-3sigma isoform resulted in a disruption of the tubulin cytoskeleton mediated by binding Tau protein. PMID: 26103986
TUBA4A mutants destabilize the microtubule network, diminishing its repolymerization capability PMID: 25374358
TUBA4A is significantly increased in spermatazoa from men with azoospermia. PMID: 24268707
The results suggested that cytoskeletal alterations in the amygdala determined by tubulin seem to be involved in the pathophysiology of drug addiction PMID: 20686780
Subunits of alphaB crystallin that exchange dynamically with the alphaB crystallin complex can interact with tubulin subunits to regulate the equilibrium between tubulin and microtubules. PMID: 20668689
increased levels in paclitaxel-resistant breast cancer cells PMID: 12054644
Some protein-protein interactions in rafts were disrupted in 3-nitrotyrosine-containing proteins, e.g. caveolin-1 was dissociated from a complex with flotillin-1 and alpha-tubulin. PMID: 15934946
Our results suggest that MIZIP might play an important role in mammalian cells by associating with tubulin and thus might provide a link between MCHR1 and tubulin functions. PMID: 16039987
These results provide the first characterisation of endogenously nitrated tubulin from human tumour samples. PMID: 16257120

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Involvement in disease

Amyotrophic lateral sclerosis 22, with or without frontotemporal dementia (ALS22)

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Families

Tubulin family

Database Links

HGNC: 12407

OMIM: 191110

KEGG: hsa:7277

STRING: 9606.ENSP00000248437

UniGene: Hs.75318

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A Comprehensive Exploration into Amyotrophic Lateral Sclerosis Exploring Neurodegeneration: A Comprehensive Guide for Researchers Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Gap junction Apoptosis

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