Product Details

Uniprot No.

Target Names

TUBG1

Alternative Names

Gamma-1-tubulin antibody; Gamma-tubulin complex component 1 antibody; GCP-1 antibody; TBG1_HUMAN antibody; TUBG antibody; TUBG1 antibody; TUBGCP1 antibody; Tubulin gamma-1 chain antibody; Tubulin, gamma 1 antibody; Tubulin, gamma polypeptide antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from the C-terminal region of Human Tubulin γ.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, IHC, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:5000

Protocols

Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

Gene References into Functions

CCT actively promotes the formation of conformationally different aggregates of gamma-tubulin, a non-amyloidogenic CCT client protein, which are mediated by specific CCT-gamma-tubulin interactions. PMID: 29339327
that in the face of predominant gamma-tubulin-1 expression, the accumulation of gamma-tubulin-2 in mature neurons and neuroblastoma cells during oxidative stress may denote a prosurvival role of gamma-tubulin-2 in neurons PMID: 28119396
All GCPs are incorporated into the helix via lateral interactions between their N-terminal domains, whereas the C-terminal domains mediate longitudinal interactions with gamma-tubulin. PMID: 27660388
While binding to gamma-tubulin may help target APC to the site of microtubule nucleation complexes, additional C-terminal sequences of APC are required to stimulate and stabilize microtubule growth. PMID: 26556314
Results show that CUL4A- and CUL4B-mediated polyubiquitination of gamma-tubulin for its degradation. PMID: 25542213
In this study, the molecular basis of interaction between two lateral gamma-tubulin units within the gamma-tubulin ring complex were elucidated by making extensive use of molecular mechanics and molecular dynamics techniques. PMID: 25031076
Gamma tubulin expression was associated with patients' age, astrocytoma grade, respectability, patient survival and performance. These factors may be important prognostic indicators for patients with astrocytomas. PMID: 24620973
Recombinant gamma-tubulin can be phosphorylated by Cdk1-cyclin B or Brsk1 and dephosphorylated by Ppp4c-R2-R3A in vitro. PMID: 23966160
Nek9 phosphorylates NEDD1 on Ser377 driving its recruitment and thereby that of gamma-tubulin to the centrosome in mitotic cells. PMID: 22818914
Our results reveal for the first time an increased expression of TUBG1 and TUBG2 in lung cancer PMID: 22806905
overexpression of Cdk1 or BRCA1 greatly expands the gamma-tubulin coating of microtubules, suggesting that the microtubule-bound gamma-tubulin is involved in DNA damage response. PMID: 21951856
in transformed as well as in non-transformed interphase mammalian cells gamma-tubulin is present not only in cytoplasm but also in nuclei and nucleoli where it can be translocated during mitosis PMID: 21465471
These results thus report for the first time the identification of Cep70 as an important centrosomal protein that interacts with gamma-tubulin and underscore its critical role in the regulation of mitotic spindle assembly. PMID: 21795687
The data presented here indicate that gamma-tubulin has a profound relationship with actin filament dynamics PMID: 21473851
a direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome PMID: 20224777
the 2.7-A crystal structure of human gamma-tubulin bound to GTP-gammaS (a non-hydrolysable GTP analogue) PMID: 15917813
analysis of human ninein isoforms that are regulated by centrosomal targeting signals and bind to gamma-tubulin PMID: 17102634
Data suggest that aberrant expression and interactions of betaIII-tubulin and gamma-tubulin may be linked to malignant changes in glial cells. PMID: 17406983
GSK-3beta regulates the localization of gamma-tubulin ring complex, including GCP5, to the spindle poles, thereby controlling the formation of proper mitotic spindles PMID: 18316369
The axial elastic modulus of tubulin diminishes as the length of the monomer increases. PMID: 18621829
Increased expression of centrosomal TUBG in atypical ductal hyperplasia and carcinoma of the breast is reported. PMID: 19215229
Stability of the small gamma-tubulin complex (gamma tubulin/GCP2/GCP3) requires HCA66, a protein of the centrosome and the nucleolus. PMID: 19299467
Data suggest that sequential phosphorylation of Nedd1 by Cdk1 and Plk1 plays a role in targeting gamma tubulin ring complex to the centrosome by promoting the interaction of Nedd1 with the gammaTuRC component gamma-tubulin, during mitosis. PMID: 19509060
Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components PMID: 19543530
SADB kinase activity controls centrosome homeostasis by regulating phosphorylation of gamma-tubulin. PMID: 19648910