Tri-Methyl-Histone H3 (Lys27) Antibody

Datasheet
Code CSB-PA877760
Size US$360
Image
  • Western blot analysis of extracts from HT29 and C6 cells using Histone H3 (Tri-Methyl-Lys27) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3 (Tri-Methyl-Lys27) Antibody.
  • Immunofluorescence staining of methanol-fixed MEF cells using Histone H3 (Tri-Methyl-Lys27) Antibody .

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) HIST1H3A Polyclonal antibody
Uniprot No. P68431
Target Names HIST1H3A
Alternative Names H3/a, H3/c, H3/d, H3/f, H3/h
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around Tri-Methylation site of lysine 27(A-R-K(tri-methyl)-S-A) derived from Human Histone H3.
Immunogen Species Homo sapiens (Human)
Clonality Polyclonal
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy u
Concentration It differs from different batches. Please contact us to confirm it.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications ELISA,WB,IHC,IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IHC 1:50-1:100
IF 1:100-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Involvement in disease Glioma (GLM)
Subcellular Location Nucleus, Chromosome
Protein Families Histone H3 family
Database Links

HGNC: 4766

OMIM: 137800

KEGG: hsa:8350

STRING: 9606.ENSP00000444823

UniGene: Hs.132854

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