Product Details

Uniprot No.

P0CG47

Target Names

UBB

Alternative Names

UBB antibody; UBB_HUMAN antibody; Ubiquitin antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein of Human UBB

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:10-1:25

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function	Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Gene References into Functions	This study demonstrated that the Deposition of mutant ubiquitin in parkinsonism-dementia complex of Guam. PMID: 29122008 The polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB have been characterized. PMID: 27861798 The C-terminal five residues of Ub, RLRGG, are responsible for the interaction with the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease. PMID: 27245450 A new crystallographic structure of human ubiquitin solved from crystals grown in the presence of magnesium. PMID: 26750481 Data suggest that both human ubiquitin and HFBII (hydrophobin-II from Trichoderma reesei) exhibit a critical surface hydration level (or effective hydrophobic interface at the surface) at which percolation transition of water network occurs. PMID: 25204743 UbB was significantly increased in prolonged Trichostatin A-selected HeLa cells and it played a key role in the maintenance of cervical cancer stem-like cells PMID: 24367661 downregulation of ubiquitin through Ubb-KD is a potential anti-cancer treatment by inhibiting ubiquitination at multiple sites related to oncogenic pathways and by weakening the ability of cancer cells to overcome increased stress. PMID: 24022007 A significant decrease in amyloid beta deposition and plaque formation suggests a role for the ubiquitin-proteasome system in the amyloid pathology of Alzheimer's disease. PMID: 22797007 age-dependent accumulation of Ubb(+1) , and how Ubb(+1) -mediated proteasome inhibition may contribute to Alzheimer's disease. [review] PMID: 22082077 Studies indicate that biomedical research on ubiquitin moves into translational research and drug discovery. PMID: 21544573 Studies indicate that DUBs recycle ubiquitin by processing polyubiquitin chains to generate free ubiquitin, and can be regulated by ubiquitination or phosphorylation. PMID: 21480003 analysis of orexin receptor 1 and 2 -arrestin-ubiquitin complexes PMID: 21378163 The results of this study demonstrated that the UBB mutation caused the subtle defect in spatial reference memory formation, caused by a decrease in forebrain proteasome activity. PMID: 21059367 Studies indicate that indicating that the inhibition of the ubiquitin-proteasome system could be used as a novel approach for cancer therapy. PMID: 20491623 Results suggest that the interaction between E2-25K and UBB(+1) is critical for the synthesis and accumulation of UBB(+1)-anchored polyubiquitin, which results in proteasomal inhibition and neuronal cell death. PMID: 20826778 Molecular misreading of the ubiquitin B gene and hepatic mallory body formation. PMID: 12055595 expression of UBB+1 causes proteasome inhibition and induces expression of heat-shock proteins; although UBB+1-expressing cells have a compromised ubiquitin-proteasome system, they are protected against oxidative stress PMID: 12871580 The temporal localization of frame-shift ubiquitin-B and amyloid precursor protein, and complement proteins in the brain of non-demented control patients with increasing Alzheimer's disease pathology. PMID: 12893422 In sporadic inclusion-body myositis, UBB+1 may be pathogenic by inhibiting proteasome, thereby promoting accumulation of cytotoxic misfolded amyloid-beta and phosphorylated-tau. PMID: 15452314 UBB+1, a mutant form of ubiquitin was present in the majority of NFTs, whereas co-existence of alpha-synuclein and UBB+1 was found in only a few neurons in cases of combined multiple system atrophy and Alzheimer's disease. PMID: 17237936 K63-polyubiquitination guards against chemical carcinogenesis by preventing mutagenesis and thus contributing to genomic stability PMID: 17395554 UBB(+1), at low expression levels, is efficiently degraded by the proteasome, but at high levels, the proteasome failed to degrade UBB(+1), causing its accumulation. PMID: 17405812 analyse the expression of mutant ubiquitin (UBB+1), in muscle biopsies from patients suffering from myotilinopathy and desminopathy PMID: 17931355 The expression a mutant human UBB analogous (UB14) in yeast markedly enhanced cellular susceptibility to toxic protein aggregates. PMID: 19214209 Show More Hide All
Subcellular Location	[Ubiquitin]: Cytoplasm. Nucleus.
Protein Families	Ubiquitin family
Database Links	HGNC: 12463 OMIM: 191339 KEGG: hsa:7314 STRING: 9606.ENSP00000304697 UniGene: Hs.356190

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Check Code：

Code	CSB-PA179850
Size	US$299 How to order?
Image	The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA179850(UBB Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA179850(UBB Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-5: Mouse pancreas tissue, Hela cells, mouse liver tissue, A549 cells, 293T cells, Primary antibody: CSB-PA179850(UBB Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
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UBB Antibody

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Target Background

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