UCHL3 Antibody

Datasheet

Code	CSB-PA998471
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA998471(UCHL3 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA998471(UCHL3 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-4: 293T, K562, Jurkat and 231 cell, Primary antibody: CSB-PA998471(UCHL3 Antibody) at dilution 1/300 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
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Product Details
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Target Background

Product Details

Uniprot No.

P15374

Target Names

UCHL3

Alternative Names

Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody; Ubiquitin thioesterase L3 antibody; Ubiquitin thiolesterase antibody; Ubiquitin thiolesterase L3 antibody; UCH L3 antibody; UCH-L3 antibody; UCHL3 antibody; UCHL3_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Full length fusion protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:25-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.

Gene References into Functions

The authors find that UCHL3 regulates COPS5-dependent deneddylation of Cullin1, which is an essential component of SCF(beta-TrCP) complex and associated with SCF(beta-TrCP) activities. The authors further demonstrate that UCHL3 upregulates the levels of SCF(beta-TrCP) substrates including IFN-I receptor IFNAR1, which enhances IFN-I mediated signaling pathway and antiviral activity. PMID: 28583475
Study demonstrated positive correlations between the level and activity of UCHL3 and sperm characteristics and function suggesting that UCHL3 may play a role in male infertility. PMID: 27780264
Using a series of engineered protein substrates, which are similar in size yet differ in secondary structure, we demonstrate that thermal stability is a key factor that significantly affects UCH-L3 hydrolysis. PMID: 25369561
UCH-L3 is a novel regulator of epithelial-mesenchymal transition and cell migration in prostate cancer cells. PMID: 25194810
Data identified of UCHL3 as an essential deubiquitinase in adipogenesis. PMID: 22679485
impaired UCH-L3 function may contribute to the accumulation of full length UBB(+1) in various pathologie PMID: 21762696
1.45 A resolution crystal structure of human UCH-L3 in complex with the inhibitor ubiquitin vinylmethylester, an inhibitor that forms a covalent adduct with the active site cysteine of ubiquitin-specific proteases PMID: 15531586
Upregulation of UCH-L3 is associated with Uterine Cervical Neoplasms PMID: 16402389
Substrate filtering by the active site crossover loop in UCHL3 revealed by sortagging and gain-of-function mutations. PMID: 19047059
These results indicate that mono-Ub and Ub dimers may regulate the enzymatic functions of UCH-L1 and UCH-L3, respectively, in vivo. PMID: 19154770
Untangling the folding mechanism of the 5(2)-knotted protein UCH-L3 PMID: 19476499
identification and the sequence-specific assignments for 186 out of the 218 UCH-L3 (230 less 12 prolines) backbone 15N and amide proton resonances PMID: 19636824

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Subcellular Location

Cytoplasm.

Protein Families

Peptidase C12 family

Tissue Specificity

Highly expressed in heart, skeletal muscle, and testis.

Database Links

HGNC: 12515

OMIM: 603090

KEGG: hsa:7347

STRING: 9606.ENSP00000366819

UniGene: Hs.162241

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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