ULK2 Antibody

Datasheet

Code	CSB-PA811629LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate All lanes: ULK2 antibody at 2.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 113 kDa Observed band size: 113 kDa IHC image of CSB-PA811629LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA811629LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ULK2 Polyclonal antibody

Uniprot No.

Q8IYT8

Target Names

ULK2

Alternative Names

ATG1B antibody; KIAA0623 antibody; Serine/threonine protein kinase ULK2 antibody; Serine/threonine-protein kinase ULK2 antibody; ULK2 antibody; ULK2_HUMAN antibody; Unc 51 (C. elegans) like kinase 2 antibody; Unc 51 like autophagy activating kinase 2 antibody; Unc 51 like kinase 2 antibody; Unc-51-like kinase 2 antibody; Unc51.2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Serine/threonine-protein kinase ULK2 protein (211-276AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ULK2 Antibody (Product code: CSB-PA811629LA01HU) is Non-conjugated. For ULK2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA811629LB01HU	ULK2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA811629LC01HU	ULK2 Antibody, FITC conjugated
Biotin	CSB-PA811629LD01HU	ULK2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.

Gene References into Functions

MiRNA-26b may play a key role in cell growth and death of laryngeal carcinoma through ULK2 and the PTEN/AKT pathway. PMID: 28713931
The serine/threonine kinase ULK2 binds to and phosphorylates CARMA2sh. PMID: 28230860
Knockdown of ULK2 expression significantly induced autophagy, EMT, and cell migration in lung cancer cell line. PMID: 27062295
miR-26b was down-regulated in LNCaP, DU145, C4-2 and PC-3 cells compared to the two normal prostate cells RWPE-1 and WPMY-1 except DU145 cells. This inversely correlates with ULK2 level in the same cell lines. PMID: 26920049
Kapbeta2 interacts with ULK2 through ULK2's putative PY-NLS motif, and facilitates transport from the cytoplasm to the nucleus, depending on its Ser1027 residue phosphorylation by PKA, thereby reducing autophagic activity. PMID: 26052940
Glioblastoma and glioma cell lines had low levels of ULK2 transcripts. ULK2 promoter methylation and transcript levels showed significant negative correlation. PMID: 24923441
First identification and naming of ULK2 as an ortholog of mouse ULK2. PMID: 12471243

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Subcellular Location

Cytoplasmic vesicle membrane; Peripheral membrane protein. Note=Localizes to pre-autophagosomal membrane.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily

Database Links

HGNC: 13480

OMIM: 608650

KEGG: hsa:9706

STRING: 9606.ENSP00000354877

UniGene: Hs.168762

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Service

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

mTOR signaling pathway Autophagy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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