USP30 Antibody

Code CSB-PA765106LA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human Kidney tissue using CSB-PA765106LA01HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA765106LA01HU at dilution of 1:100
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) USP30 Polyclonal antibody
Uniprot No.
Target Names
USP30
Alternative Names
Deubiquitinating enzyme 30 antibody; FLJ40511 antibody; Ub-specific protease 30 antibody; Ubiquitin carboxyl terminal hydrolase 30 antibody; Ubiquitin carboxyl-terminal hydrolase 30 antibody; Ubiquitin specific peptidase 30 antibody; Ubiquitin specific processing protease 30 antibody; Ubiquitin thioesterase 30 antibody; Ubiquitin thiolesterase 30 antibody; Ubiquitin-specific-processing protease 30 antibody; UBP30_HUMAN antibody; usp30 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 30 protein (101-400AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The USP30 Antibody (Product code: CSB-PA765106LA01HU) is Non-conjugated. For USP30 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA765106LB01HU USP30 Antibody, HRP conjugated ELISA
FITC CSB-PA765106LC01HU USP30 Antibody, FITC conjugated
Biotin CSB-PA765106LD01HU USP30 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The polyclonal antibody against USP30 was created in rabbits through immunization with recombinant human USP30 protein containing amino acids 101-400. This USP30 polyclonal antibody is only reactive with USP30 protein from human samples. The deubiquitinase enzyme USP30 is responsible for the regulation of protein turnover and maintenance of mitochondrial function.

The USP30 antibody has been purified via protein G affinity chromatography, achieving more than 95% purity. Its validation in ELISA and IHC applications has further enhanced its versatility in experimental settings.

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Target Background

Function
Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2.
Gene References into Functions
  1. the role of USP30 in the dynamic networks of mitochondrial quality control and its physiological implications. PMID: 29178074
  2. Here the authors report crystal structures of human USP30 bound to monoubiquitin and Lys6-linked diubiquitin, which explain how USP30 achieves Lys6-linkage preference through unique ubiquitin binding interfaces. PMID: 28945249
  3. show that USP30 delays mitophagy by delaying PARK2 recruitment to the mitochondria during mitophagy PMID: 25915564
  4. These results provide the first evidence for a fundamental role of USP30 in determining the threshold for mitochondrial cell death and suggest USP30 as a potential target for combinatorial anti-cancer therapy. PMID: 25739811
  5. The mechanism by which parkin, an E3 ubiquitin ligase, and USP30, a mitochondrion-localized deubiquitylase, regulate mitophagy, was investigated. PMID: 25621951
  6. reducing USP30 activity enhances mitochondrial degradation in neurons PMID: 24896179
  7. USP30 participates in the maintenance of mitochondrial morphology, a finding that provides new insight into the cellular function of deubiquitination. PMID: 18287522

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Subcellular Location
Mitochondrion outer membrane.
Protein Families
Peptidase C19 family
Tissue Specificity
Expressed in skeletal muscle, pancreas, liver and kidney.
Database Links

HGNC: 20065

OMIM: 612492

KEGG: hsa:84749

STRING: 9606.ENSP00000257548

UniGene: Hs.486434

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