VMP1 Antibody

Datasheet

Code	CSB-PA292260
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA292260(VMP1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA292260(VMP1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 80 μg, Lane: 293T cell, Primary antibody: CSB-PA292260(VMP1 Antibody) at dilution 1/800 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q96GC9

Target Names

VMP1

Alternative Names

VMP1; TDC1; TMEM49; HSPC292; Vacuole membrane protein 1; Transmembrane protein 49

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide of Human VMP1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:25-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Phospholipid scramblase involved in lipid homeostasis and membrane dynamics processes. Has phospholipid scramblase activity toward cholesterol and phosphatidylserine, as well as phosphatidylethanolamine and phosphatidylcholine. Required for autophagosome formation: participates in early stages of autophagosome biogenesis at the endoplasmic reticulum (ER) membrane by reequilibrating the leaflets of the ER as lipids are extracted by ATG2 (ATG2A or ATG2B) to mediate autophagosome assembly. Regulates ATP2A2 activity to control ER-isolation membrane contacts for autophagosome formation. In addition to autophagy, involved in other processes in which phospholipid scramblase activity is required. Modulates ER contacts with lipid droplets, mitochondria and endosomes. Plays an essential role in formation of cell junctions. Upon stress such as bacterial and viral infection, promotes formation of cytoplasmic vacuoles followed by cell death. Involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis.; (Microbial infection) Host factor required for infection by all flaviviruses tested such as Zika virus and Yellow fever virus. Probably required post-entry of the virus to facilitate the ER membrane remodeling necessary to form replication organelles.

Gene References into Functions

It has been concluded that VMP1-mediated autophagy cooperated with Kras to promote pancreatic ductal adenocarcinoma initiation. PMID: 27415425
VMP1 modulates SERCA2 activity to control endoplasmic reticulum contacts for autophagosome formation. PMID: 28890335
We hypothesize that in autophagy VMP1 is required for the correct morphogenesis of the omegasome by regulating MCS at the site of autophagosome formation. PMID: 27861594
expression of TMEM49 seems to be associated with the apoptosis and metastasis up signaling pathways of ovarian cancer, and suppression of its expression can inhibit cancer cell growth and metastasis PMID: 27023910
Study indicated that the expression of VMP1 was down-regulated and negatively correlated to the prognosis of colorectal cancer. PMID: 26328607
In colorectal cancer cells, VMP1-dependent autophagy appears to be pro-survival rather than pro-cell death. PMID: 24365149
in patients with invasive ductal carcinoma of the breast, Vmp1 expression had significant influence on disease-free survival time, with Vmp1-negative patients showing poor prognosis PMID: 23710913
These results underscore a novel role for VMP1 as a potential therapeutic target for combinatorial therapies aimed at sensitizing pancreatic cancer cells to chemotherapeutic agents PMID: 23460482
The VMP1-Beclin 1 interaction regulates autophagy induction. PMID: 23316280
upregulation of Vmp1 was associated with suppression of growth and pulmonary metastases of HCC. PMID: 22971212
AKT1-GLI3-VMP1 pathway mediates KRAS oncogene-induced autophagy in cancer cells. PMID: 22535956
The expression of VMP1 had significant negative influence on the survival rate of non-small-cell lung cancer patients. PMID: 22333543
VMP1 is reduced by hypoxia, and down-regulation of VMP1 by miR-210 mediates hypoxia-induced HCC cell metastasis. PMID: 22144109
A fusion transcript (RPS6KB1-VMP1) recurrently expressed in 30% of breast cancers is associated with clinical consequences and may indicate genomic instability altering the expression of oncogenic components such as MIR21 and RPS6KB1. PMID: 21467264
Zymophagy, a novel selective autophagy pathway mediated by VMP1-USP9x-p62, prevents pancreatic cell death. PMID: 21173155
VMP1 knockdown decreased autophagy and apoptosis in gemcitabine-treated cancer cells PMID: 20299819
Endogenously expressed Vmp1 is essentially a cell membrane protein that is involved in cell-cell adhesion, invasion and metastasis. PMID: 17724469
VMP1 as a novel autophagy-related membrane protein involved in the initial steps of the mammalian cell autophagic process. PMID: 17940279
Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development PMID: 18550798

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Subcellular Location

Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

VMP1 family

Database Links

HGNC: 29559

OMIM: 611753

KEGG: hsa:81671

STRING: 9606.ENSP00000262291

UniGene: Hs.444569

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Autophagy

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