WDFY2 Antibody

Datasheet

Code	CSB-PA850405LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: WDFY2 antibody at 5.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa Immunofluorescence staining of Hela cells with CSB-PA850405LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) WDFY2 Polyclonal antibody

Uniprot No.

Q96P53

Target Names

WDFY2

Alternative Names

PROF antibody; Propeller-FYVE protein antibody; RP11 147H23.1 antibody; WD repeat and FYVE domain containing 2 antibody; WD repeat and FYVE domain-containing protein 2 antibody; WD40 and FYVE domain containing 2 antibody; WD40- and FYVE domain-containing protein 2 antibody; WDF2 antibody; Wdfy2 antibody; WDFY2_HUMAN antibody; ZFYVE22 antibody; Zinc finger FYVE domain-containing protein 22 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human WD repeat and FYVE domain-containing protein 2 protein (2-114AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The WDFY2 Antibody (Product code: CSB-PA850405LA01HU) is Non-conjugated. For WDFY2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA850405LB01HU	WDFY2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA850405LC01HU	WDFY2 Antibody, FITC conjugated
Biotin	CSB-PA850405LD01HU	WDFY2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Acts in an adapter protein-like fashion to mediate the interaction between the kinase PRKCZ and its substrate VAMP2 and increases the PRKCZ-dependent phosphorylation of VAMP2. Positively regulates adipocyte differentiation, by facilitating the phosphorylation and thus inactivation of the anti-adipogenetic transcription factor FOXO1 by the kinase AKT1. Plays a role in endosomal control of AKT2 signaling; required for insulin-stimulated AKT2 phosphorylation and glucose uptake and insulin-stimulated phosphorylation of AKT2 substrates. Participates in transferrin receptor endocytosis.

Gene References into Functions

Our results showed that WDFY2 inhibited cancer cell colony formation and migration via suppressing Akt pathway, making it a potential new therapeutic target in prostate cancer. PMID: 28653900
CDKN2D-WDFY2 fusion could be an important molecular signature for understanding and classifying sub-lineages among heterogeneous high-grade serous ovarian carcinomas. PMID: 24675677
The ProF protein partially co-localizes with EEA1 on vesicular structures and binds to the protein kinases Akt and PKCzeta/lambda (protein kinase Czeta/lambda) via its WD-repeat propeller. PMID: 16792529
WDFY2, the WD40 and FYVE domain containing protein 2, has roles in endocytosis PMID: 16873553
presence of ProF increases the PKCzeta-dependent phosphorylation of VAMP2 in vitro PMID: 17313651

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Subcellular Location

Endosome. Early endosome. Cytoplasm.

Database Links

HGNC: 20482

OMIM: 610418

KEGG: hsa:115825

STRING: 9606.ENSP00000298125

UniGene: Hs.208550

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Service

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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