YOD1 Antibody

Datasheet

Code	CSB-PA713352LA01HU
Size	US$166
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Image	Immunofluorescence staining of A549 cells with CSB-PA713352LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) YOD1 Polyclonal antibody

Uniprot No.

Q5VVQ6

Target Names

YOD1

Alternative Names

DUBA-8 antibody; DUBA8 antibody; HIN-7 antibody; HIV-1-induced protease 7 antibody; HsHIN7 antibody; OTU domain containing 2 antibody; OTU domain-containing protein 2 antibody; OTU1_HUMAN antibody; OTUD2 antibody; PRO0907 antibody; RP11-164O23.1 antibody; Ubiquitin thioesterase OTU1 antibody; yod1 antibody; YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) antibody; YOD1 OTU deubiquinating enzyme 1 homolog (yeast) antibody; YOD1 OTU deubiquinating enzyme 1; S. cerevisiae; homolog of antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ubiquitin thioesterase OTU1 protein (1-44AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The YOD1 Antibody (Product code: CSB-PA713352LA01HU) is Non-conjugated. For YOD1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA713352LB01HU	YOD1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA713352LC01HU	YOD1 Antibody, FITC conjugated
Biotin	CSB-PA713352LD01HU	YOD1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. May recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation.

Gene References into Functions

This study showed that YOD1 overexpression enhances cell migration by promoting TGF-beta3 signaling which may play an important role in lip and palate formation. PMID: 30145984
Mechanistically, YOD1 deubiquitinates ITCH, an E3 ligase of LATS, and enhances the stability of ITCH, which leads to reduced levels of LATS and a subsequent increase in the YAP/TAZ level. PMID: 28416659
Data suggest that YOD1 is a novel regulator of the Hippo pathway, and thereby a potential therapeutic target for liver cancer. PMID: 28502290
These data define that YOD1 antagonizes TRAF6/p62-dependent IL-1 signaling to NF-kappaB. PMID: 28244869
Upon damage, p97 translocates to lysosomes and there cooperates with a distinct set of cofactors including UBXD1, PLAA, and the deubiquitinating enzyme YOD1, which we term ELDR components for Endo-Lysosomal Damage Response. PMID: 27753622
activity of the p97-associated deubiquitinylase YOD1 is also required for substrate disposal PMID: 26463207
miR-373 increases proliferation by directly targeting YOD1 in cervical cancer. PMID: 25747718
The assignment of a p97-associated ubiquitin processing function to YOD1 adds to our understanding of p97's role in the dislocation of misfolded proteins from the endoplasmic reticulum. PMID: 19818707

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Subcellular Location

Cytoplasm.

Database Links

HGNC: 25035

OMIM: 612023

KEGG: hsa:55432

STRING: 9606.ENSP00000326813

UniGene: Hs.567533

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Related pathways

Protein processing in endoplasmic reticulum

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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