ZC3H14 Antibody

Datasheet

Code	CSB-PA740793LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate All lanes: ZC3H14 antibody at 3.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 83, 66, 65, 81, 35, 33, 62, 67 kDa Observed band size: 83 kDa IHC image of CSB-PA740793LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA740793LA01HU diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ZC3H14 Polyclonal antibody

Uniprot No.

Q6PJT7

Target Names

ZC3H14

Alternative Names

FLJ11806 antibody; Mammalian suppressor of tau pathology-2 antibody; MGC26892 antibody; MSUT-2 antibody; Nuclear protein UKp68 antibody; NY REN 37 antibody; NYREN 37 antibody; Renal carcinoma antigen NY REN 37 antibody; Renal carcinoma antigen NY-REN-37 antibody; Renal carcinoma antigen NYREN 37 antibody; suppressor of tau 2, C. elegans, homolog of antibody; SUT2 antibody; UKp68 antibody; ZC3H14 antibody; ZC3HE_HUMAN antibody; Zinc finger CCCH domain-containing protein 14 antibody; Zinc finger CCCH type containing 14 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Zinc finger CCCH domain-containing protein 14 protein (176-306AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ZC3H14 Antibody (Product code: CSB-PA740793LA01HU) is Non-conjugated. For ZC3H14 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA740793LB01HU	ZC3H14 Antibody, HRP conjugated	ELISA
FITC	CSB-PA740793LC01HU	ZC3H14 Antibody, FITC conjugated
Biotin	CSB-PA740793LD01HU	ZC3H14 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Involved in poly(A) tail length control in neuronal cells. Binds the polyadenosine RNA oligonucleotides.

Gene References into Functions

ATP5G1 turnover increases upon depletion of ZC3H14, double knockdown of ZC3H14 and the nonsense-mediated decay factor, UPF1, rescues ATP5G1 transcript levels. Furthermore, fractionation reveals an increase in the amount of ATP5G1 pre-mRNA that reaches the cytoplasm when ZC3H14 is depleted and that ZC3H14 binds to ATP5G1 pre-mRNA in the nucleus. PMID: 27563065
we show here that human ZC3H14 can functionally substitute for dNab2 in fly neurons and can rescue defects in development and locomotion that are present in dNab2 null flies PMID: 24671764
report a intellectual disability disease locus on chromosome 14q31.3 corresponding to mutation of the ZC3H14 gene that encodes a conserved polyadenosine RNA binding protein PMID: 21734151
MSUT2 levels may influence neuronal vulnerability to tau toxicity and aggregation. PMID: 21355046
New neuroprotective strategies targeting MSUT-2 that may be effective in modulating tau neurotoxicity in human tauopathy disorders. PMID: 20658987
these proteins are members of an evolutionarily conserved family of poly(A) RNA binding proteins PMID: 17630287
multiple transcripts encoding several ZC3H14 isoforms exist in vivo PMID: 19303045

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Involvement in disease

Mental retardation, autosomal recessive 56 (MRT56)

Subcellular Location

Nucleus speckle.; [Isoform 1]: Nucleus speckle.; [Isoform 3]: Nucleus speckle.; [Isoform 6]: Cytoplasm.

Protein Families

ZC3H14 family

Tissue Specificity

Isoform 1 and isoform 6 are expressed in fetal and adult brain. Isoform 1 and isoform 6 are expressed in fetal and adult temporal lobe.

Database Links

HGNC: 20509

OMIM: 613279

KEGG: hsa:79882

STRING: 9606.ENSP00000251038

UniGene: Hs.684046

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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