deoD Antibody

Datasheet

Code	CSB-PA18357A0Rb
Size	US$166
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Image	Western blot All lanes: deoD antibody at 2µg/ml + DH5a whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli deoD Polyclonal antibody

Uniprot No.

P0ABP8

Target Names

deoD

Alternative Names

deoD antibody; pup antibody; b4384 antibody; JW4347 antibody; Purine nucleoside phosphorylase DeoD-type antibody; PNP antibody; EC 2.4.2.1 antibody; Inosine phosphorylase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type protein (2-239AA)

Immunogen Species

Escherichia coli

Conjugate

Non-conjugated

The deoD Antibody (Product code: CSB-PA18357A0Rb) is Non-conjugated. For deoD Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA18357B0Rb	deoD Antibody, HRP conjugated	ELISA
FITC	CSB-PA18357C0Rb	deoD Antibody, FITC conjugated
Biotin	CSB-PA18357D0Rb	deoD Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the reversible phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Acts on 6-amino and 6-oxopurines including deoxyinosine, deoxyguanosine, deoxyadenosine, adenosine, guanosine, and inosine. Does not act on xanthosine. May also catalyze a phosphate-dependent transfer of the pentose moiety from one purine base to another.

Gene References into Functions

These studies also indicate an important role of the phenyl ring of Phe159 for FRET in the wild-type purine nucleoside phosphorylase, which does not exist in the Ala159 mutant, and for the effective association of PNP with formycin A . PMID: 25754135
Data suggest that in active sites of purine nucleoside phosphorylase complexed with phosphate and formycin A, recombinant enzyme exhibits unexpected binding site for phosphate and exhibits stoichiometry of two phosphate molecules per enzyme subunit. PMID: 22569248
The deoD gene was cloned from Escherichia coli MG1665 & overexpressed in E. coli BL 21(DE3). PMID: 22113014
in solution similar to the crystal, E. coli PNP is a hexameric molecule and previous suggestions for coexistence of two oligomeric forms are incorrect. PMID: 16751611
Role of ionization of the phosphate cosubstrate on phosphorolysis by purine nucleoside phosphorylase (PNP) PMID: 17639373

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Protein Families

PNP/UDP phosphorylase family

Database Links

KEGG: ecj:JW4347

STRING: 316385.ECDH10B_4542

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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