lecA Antibody, Biotin conjugated

Datasheet

Code	CSB-PA313124HD01EZX
Size	US$166
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) lecA Polyclonal antibody

Uniprot No.

Q05097

Target Names

lecA

Alternative Names

lecA antibody; pa1L antibody; PA2570 antibody; PA-I galactophilic lectin antibody; PA-IL antibody; Galactose-binding lectin antibody

Raised in

Rabbit

Species Reactivity

Pseudomonas aeruginosa

Immunogen

Recombinant Pseudomonas aeruginosa PA-I galactophilic lectin protein (2-122AA)

Immunogen Species

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Conjugate

Biotin

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.

Gene References into Functions

The crystal structure of LecA/melibiose complex shows a secondary sugar binding site with glucose bound. PMID: 24123124
Study established the structural features of PA-IL (LecA} in complex with the three disaccharides by docking and molecular dynamics simulations. PMID: 20410292
It is concluded that the carbohydrate recognizing site of the lectin can have a binding requirement of only one saccharide. PMID: 16542817
Results describe the crystal structure of P. aeruginosa lectin I complexed to alphaGal1-3betaGal1-4Glc trisaccharide at 1.9-A resolution and reveal how the second galactose residue makes specific contacts with the protein surface. PMID: 18762193
The results demonstrate that there is a relationship between the LecA and LecB lectins and the pathogenicity of P. aeruginosa. PMID: 19237519

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Subcellular Location

Cytoplasm. Periplasm. Cell surface. Note=Low exposure on the cell surface.

Protein Families

LecA/PllA lectin family

Database Links

KEGG: pae:PA2570

STRING: 208964.PA2570

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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