Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin(ply)

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Code CSB-BP314690FMW
Lead Time 3-7 business days
Product Type Recombinant Protein
Size Pls inquiry other sizes
Uniprot No. P0C2J9
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Relevance Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • Based on the SEQUEST from database of Baculovirus host and target protein, the LC-MS/MS Analysis result of CSB-BP314690FMW could indicate that this peptide derived from Baculovirus-expressed Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) ply.

  • Based on the SEQUEST from database of Baculovirus host and target protein, the LC-MS/MS Analysis result of CSB-BP314690FMW could indicate that this peptide derived from Baculovirus-expressed Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) ply.

Storage Buffer Tris-based buffer,50% glycerol
Species Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
Research Area Others
Source Baculovirus
Gene Names ply
Protein Names Thiol-activated cytolysin
Expression Region 2-471aa
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Product Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Mol. Weight 56.8 kDa
Protein Description Full Length of Mature Protein
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Function Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation (By similarity).
Subcellular Location Secreted, Host cell membrane, Multi-pass membrane protein
Protein Families Thiol-activated cytolysin family
Database Links

KEGG: spn:SP_1923

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