Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ply

Uniprot No.

P0C2J9

Research Area

Others

Alternative Names

ply; SP_1923; Pneumolysin; Ply; Thiol-activated cytolysin

Species

Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

Source

Baculovirus

Expression Region

2-471aa

Target Protein Sequence

ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

56.8 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The synthesis of Recombinant S.pneumoniae serotype 4 ply protein began with the isolation of ply gene from the native genome. The next step was to amplify the ply gene by inserting the ply gene into an expression vector and then introducing the vector into the selected host cell, Baculovirus. Significant amounts of recombinant S.pneumoniae serotype 4 ply protein were produced by the host only when expression genes were added. For the purpose of protein purification, the vector contains N-terminal 10xHis tag & C-terminal Myc tag to the ply DNA sequence. The purity of this protein is 90%+ determined by SDS-PAGE.

ply (also known as SP_1923) is a gene encoding a protein named pneumolysin (ply) in Streptococcus pneumoniae serotype 4. The protein encoded by this gene is also known as thiol-activated cytolysin and belongs to Thiol-activated cytolysin family. The molecular weight of this protein is 53 kDa composed of 471 amino acids. It has a multiple biological activity, including the ability to lyse and interfere with the function of cells and soluble molecules of the immune system.

Target Background

Function

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Subcellular Location

Secreted. Secreted, cell wall. Host cell membrane; Multi-pass membrane protein.

Protein Families

Thiol-activated cytolysin family

Database Links

KEGG: spn:SP_1923

Code	CSB-BP314690FMW
Abbreviation	Recombinant Streptococcus pneumoniae serotype 4 ply protein
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Baculovirus host and target protein, the LC-MS/MS Analysis result of CSB-BP314690FMW could indicate that this peptide derived from Baculovirus-expressed Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) ply. Based on the SEQUEST from database of Baculovirus host and target protein, the LC-MS/MS Analysis result of CSB-BP314690FMW could indicate that this peptide derived from Baculovirus-expressed Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) ply.
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Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply)

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