Recombinant Arabidopsis thaliana Auxin-responsive protein IAA17 (IAA17)

Code CSB-YP308638DOA
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Source Yeast
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Code CSB-EP308638DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP308638DOA
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Source Baculovirus
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Code CSB-MP308638DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IAA17
Uniprot No.
Alternative Names
IAA17; AXR3; At1g04250; F19P19.31; Auxin-responsive protein IAA17; Auxin response 3; Indoleacetic acid-induced protein 17
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-229
Target Protein Sequence
MMGSVELNLR ETELCLGLPG GDTVAPVTGN KRGFSETVDL KLNLNNEPAN KEGSTTHDVV TFDSKEKSAC PKDPAKPPAK AQVVGWPPVR SYRKNVMVSC QKSSGGPEAA AFVKVSMDGA PYLRKIDLRM YKSYDELSNA LSNMFSSFTM GKHGGEEGMI DFMNERKLMD LVNSWDYVPS YEDKDGDWML VGDVPWPMFV DTCKRLRLMK GSDAIGLAPR AMEKCKSRA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.
Gene References into Functions
  1. the results indicate that AtIAA17 is a positive modulator of natural leaf senescence and provides direct link between melatonin and AtIAA17 in the process of natural leaf senescence in Arabidopsis PMID: 25324183
  2. icu6 is a novel mutant allele of AXR3 that causes severe leaf incurvature, as well as enhanced responsiveness to auxin and growth defects in the adaxial domain of leaves. PMID: 20739302
  3. AXR3/IAA17 might be involved in the brassinosteroid (BR) signaling pathway, suggesting an intersection node of BR-auxin signaling in root development PMID: 16636440
  4. PAX1 influences auxin response via its effects on AXR3 expression. PMID: 17430601
Subcellular Location
Nucleus.
Protein Families
Aux/IAA family
Database Links

KEGG: ath:AT1G04250

STRING: 3702.AT1G04250.1

UniGene: At.20252

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