Recombinant Arabidopsis thaliana Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)

Code CSB-YP326578DOA
MSDS
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Source Yeast
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Code CSB-EP326578DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326578DOA
MSDS
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Source Baculovirus
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Code CSB-MP326578DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GAPC1
Uniprot No.
Alternative Names
GAPC1; GAPC; GAPDH; At3g04120; T6K12.26Glyceraldehyde-3-phosphate dehydrogenase GAPC1; cytosolic; EC 1.2.1.12; NAD-dependent glyceraldehydephosphate dehydrogenase C subunit 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-338
Target Protein Sequence
MADKKIRIGI NGFGRIGRLV ARVVLQRDDV ELVAVNDPFI TTEYMTYMFK YDSVHGQWKH NELKIKDEKT LLFGEKPVTV FGIRNPEDIP WAEAGADYVV ESTGVFTDKD KAAAHLKGGA KKVVISAPSK DAPMFVVGVN EHEYKSDLDI VSNASCTTNC LAPLAKVIND RFGIVEGLMT TVHSITATQK TVDGPSMKDW RGGRAASFNI IPSSTGAAKA VGKVLPALNG KLTGMSFRVP TVDVSVVDLT VRLEKAATYD EIKKAIKEES EGKLKGILGY TEDDVVSTDF VGDNRSSIFD AKAGIALSDK FVKLVSWYDN EWGYSSRVVD LIVHMSKA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism. Required for full fertility. Involved in response to oxidative stress by mediating plant responses to abscisic acid (ABA) and water deficits through the activation of PLDDELTA and production of phosphatidic acid (PA), a multifunctional stress signaling lipid in plants. Associates with FBA6 to the outer mitochondrial membrane, in a redox-dependent manner, leading to binding and bundling of actin. Actin binding and bundling occurs under oxidizing conditions and is reversible under reducing conditions. May be part of a redox-dependent retrograde signal transduction network for adaptation upon oxidative stress. Binds DNA in vitro.
Gene References into Functions
  1. the redox-dependent localization of GapC1 and GapC2 in the different cellular subcompartments and their interactions with VDAC3 and Trx-h3 PMID: 30189844
  2. The E3 ubiquitin-ligase SEVEN IN ABSENTIA like 7 mono-ubiquitinates GAPC1 in vitro and is required for its nuclear localization. PMID: 26582368
  3. After perception of bacterial flagellin, GAPC1 dynamically responded with a significant increase in size of fluorescent puncta and enhanced nuclear accumulation PMID: 25918875
  4. FERONIA controls leaf starch accumulation by interacting with GAPC1. PMID: 26232644
  5. GAPC levels play important roles in the overall cellular production of reductants, energy, and carbohydrate metabolites and that GAPC levels are directly correlated with seed oil accumulation. PMID: 24989043
  6. Glycolytic GAPC1, through its high sensitivity to the cellular redox state, may play a role in oxidative stress signaling or protection in plants. PMID: 23569110
  7. Results indicate that the H(2)O(2)-promoted interaction of GAPC and PLDdelta may provide a direct connection between membrane lipid-based signaling, energy metabolism and growth control in the plant response to ROS and water stress. PMID: 22589465
  8. The addition of GSSG, and much more efficiently of S-nitrosoglutathione, was shown to inactivate the enzymes GapC1 and GapC2 from Arabidopsis thaliana, spinach, yeast and rabbit muscle. [GapC1] PMID: 18298409

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Glyceraldehyde-3-phosphate dehydrogenase family
Tissue Specificity
Expressed in leaves, stems and siliques and at lower levels in roots and flowers.
Database Links

KEGG: ath:AT3G04120

STRING: 3702.AT3G04120.1

UniGene: At.22963

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