Recombinant Arabidopsis thaliana Mitochondrial outer membrane protein porin 3 (VDAC3)

Code CSB-YP882912DOA
MSDS
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Source Yeast
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Code CSB-EP882912DOA
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Source E.coli
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Code CSB-EP882912DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP882912DOA
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Source Baculovirus
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Code CSB-MP882912DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VDAC3
Uniprot No.
Alternative Names
VDAC3; HSR2; At5g15090; F2G14.210Mitochondrial outer membrane protein porin 3; Protein HYPERSENSITIVE RESPONSE 2; Athsr2; Voltage-dependent anion-selective channel protein 3; AtVDAC3; VDAC-3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
2-274
Target Protein Sequence
VKGPGLYTE IGKKARDLLY RDYQGDQKFS VTTYSSTGVA ITTTGTNKGS LFLGDVATQV KNNNFTADVK VSTDSSLLTT LTFDEPAPGL KVIVQAKLPD HKSGKAEVQY FHDYAGISTS VGFTATPIVN FSGVVGTNGL SLGTDVAYNT ESGNFKHFNA GFNFTKDDLT ASLILNDKGE KLNASYYQIV SPSTVVGAEI SHNFTTKENA ITVGTQHALD PLTTVKARVN NAGVANALIQ HEWRPKSFFT VSGEVDSKAI DKSAKVGIAL ALKP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
Gene References into Functions
  1. VDAC3 has a large conductance and is open at or around 0 mV and closes at positive or negative voltages.VDAC3 exhibits both fast and slow kinetics. PMID: 25686492
  2. Alternative cleavage and polyadenylation produces two major isoforms of VDAC3; the longest mRNA is targeted to the mitochondrial surface PMID: 24889622
  3. AtVDAC3 is in abundance in plasma membrane. PMID: 22294207
  4. VDAC3 expression is low compared with other VDAC members and its knockout mutant does not show any clear phenotype. PMID: 21705391
  5. The plant-specific kinesin, KP1, specifically interacts with VDAC3 on the mitochondrial outer membrane and that both KP1 and VDAC3 regulate aerobic respiration during seed germination at low temperature. PMID: 21406623

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Subcellular Location
Cell membrane. Mitochondrion outer membrane.
Protein Families
Eukaryotic mitochondrial porin (TC 1.B.8.1) family
Tissue Specificity
Expressed in leaf tips, anthers and stigma.
Database Links

KEGG: ath:AT5G15090

STRING: 3702.AT5G15090.1

UniGene: At.24654

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