Recombinant Archaeoglobus fulgidus DNA polymerase II small subunit (polB)

Code CSB-YP523071DOC
MSDS
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Source Yeast
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Code CSB-EP523071DOC
MSDS
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Source E.coli
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Code CSB-EP523071DOC-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP523071DOC
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP523071DOC
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
polB
Uniprot No.
Alternative Names
polB; AF_1790DNA polymerase II small subunit; Pol II; EC 2.7.7.7; Exodeoxyribonuclease small subunit; EC 3.1.11.1
Species
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Expression Region
1-488
Target Protein Sequence
MVIKNIDAAT VAKKFLVRGY NIDPKAAELI CKSGLFSDEL VDKICRIANG GFIIEKSVVE EFLRNLSNLK PATLTPRPEE RKVEEVKASC IALKVIKDIT GKSSCQGNVE DFLMYFNSRL EKLSRIIRSR VNTTPIAHAG KVRGNVSVVG MVNEVYERGD KCYIRLEDTT GTITCVATGK NAEVARELLG DEVIGVTGLL KGSSLYANRI VFPDVPINGN GEKKRDFYIV FLSDTHFGSK EFLEKEWEMF VRWLKGEVGG KKSQNLAEKV KYIVIAGDIV DGIGVYPGQE DDLAISDIYG QYEFAASHLD EIPKEIKIIV SPGNHDAVRQ AEPQPAFEGE IRSLFPKNVE HVGNPAYVDI EGVKVLIYHG RSIDDIISKI PRLSYDEPQK VMEELLKRRH LSPIYGGRTP LAPEREDYLV IEDVPDILHC GHIHTYGTGF YRGVFMVNSS TWQAQTEFQK KVNLNPMPGN VAVYRPGGEV IRLRFYGE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.
Protein Families
DNA polymerase delta/II small subunit family
Database Links

KEGG: afu:AF_1790

STRING: 224325.AF1790

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