Recombinant Bovine Endothelin-1(EDN1)

Code CSB-YP007400BO
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Source Yeast
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Code CSB-EP007400BO
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Source E.coli
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Code CSB-EP007400BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007400BO
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Source Baculovirus
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Code CSB-MP007400BO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names EDN1
Uniprot No. P17322
Alternative Names EDN1Endothelin-1; ET-1; Preproendothelin-1; PPET1) [Cleaved into: Big endothelin-1]
Species Bos taurus (Bovine)
Expression Region 53-73
Target Protein Sequence CSCSSLMD KECVYFCHLD IIW
Protein Length Cytoplasmic domain
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
Gene References into Functions
  1. EDN-1 may be involved in the protection of sperm from phagocytosis by polymorphonuclear neutrophils in the bovine oviduct, supporting sperm survival until fertilization PMID: 26781611
  2. Data suggest that, in Fallopian tubes, endothelins (EDN1, EDN2, EDN3) and EDN-converting enzymes (ECE1, ECE2) are expressed in epithelial cells; EDN receptors (EDNRA, EDNRB) are present in smooth-muscle. Expression of EDN1, EDN2, and ECE2 is highest on day of ovulation. EDN/ENDR signaling appears to participate Fallopian tube function. These studies were conducted in Holstein cows. PMID: 25370848
  3. results suggest that ET-1-induced activation of proMMP-2 is mediated via cross-talk between NADPH oxidase-PKCalpha-p(38)MAPK and NFkappaB-MT1MMP signaling pathways along with a marked decrease in TIMP-2 expression in the cells PMID: 26910780
  4. We demonstrated that (i) treatment of bovine pulmonary artery smooth muscle cells with ET-1 stimulates cPLA2 activity in the cell membrane. PMID: 25399298
  5. Differential cell-specific and spatiotemporal expression of the EDN1 system and NOS in the bovine utero-placental unit may be associated with regulation of vascular and cellular functions during pregnancy. PMID: 22921266
  6. Oxidized LDL is a stimulus of ET-1 production in cultured vascular endothelial cells. PMID: 12826225
  7. Endothelin-1 decreases ethanolamine plasmalogen levels and evokes platelet-activating factor production in brain microvessels PMID: 14609525
  8. prostaglandin F2alpha, endothelin-1, and angiotensin II may interact with each other in a local positive feedback manner to activate their secretion in the regressing corpus luteum, thus accelerating and completing luteolysis PMID: 15269098
  9. LOX-1 and CD40 synergistically, but through a distinct pathway, work to induce endothelin-1 expression in endothelial cells. PMID: 15838273
  10. Elevated local expression of ET-1 and Ednra/Ednrb during the peri-ovulatory period induces the high contractile activity of the oviduct to optimize gamete transport. PMID: 15838292
  11. Suggest that the activation of a local positive feedback mechanism in the corpus luteum among ET-1, Ang II and PGF2alpha might play a functional role in the paracrine modulation of luteolytic cascade. PMID: 15838293
  12. The combined metabolic burden of homocysteine and high glucose stimulates ET-1 synthesis in bovine aortic endothelial cells via a mechanism dependent on the production of mitochondrial ROS, but may not be generalisable to all types of endothelial cells. PMID: 16410680
  13. hypoxia and endothelin-1 have roles in mediating vasa vasorum neovascularization with pulmonary artery adventitial fibroblasts PMID: 16723696
  14. The present study thus showed that neither leptin nor resistin affects the expression of endothelin-1 or adrenomedullin in bovine brain microvascular endothelial cells. PMID: 16940707
  15. Intraluteal OXT may locally modulate secretion of vasoactive substances, particularly EDN1 and PGF(2alpha) within the corpus luteum. PMID: 17287495
  16. Endothelin may be associated with final maturation of follicles. PMID: 17380043
  17. Dehydroepiandrosterone stimulates phosphorylation of FoxO1 in vascular endothelial cells via phosphatidylinositol 3-kinase- and protein kinase A-dependent signaling pathways to regulate ET-1 synthesis and secretion PMID: 18718910

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Subcellular Location Secreted.
Protein Families Endothelin/sarafotoxin family
Database Links

KEGG: bta:281137

STRING: 9913.ENSBTAP00000010649

UniGene: Bt.59529

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