Recombinant Bovine Fatty acid-binding protein, adipocyte(FABP4)

Code CSB-YP007945BO
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Source Yeast
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Code CSB-EP007945BO
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Source E.coli
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Code CSB-EP007945BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007945BO
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Source Baculovirus
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Code CSB-MP007945BO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names FABP4
Uniprot No. P48035
Alternative Names FABP4Fatty acid-binding protein; adipocyte; Adipocyte lipid-binding protein; ALBP; Adipocyte-type fatty acid-binding protein; A-FABP; AFABP; Fatty acid-binding protein 4
Species Bos taurus (Bovine)
Expression Region 2-132
Target Protein Sequence CDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPT LIISLNGGVV TIKSESTFKN TEISFKLGQE FDEITPDDRK VKSIVNLDEG ALVQVQNWDG KSTTIKRKLM DDKMVLECVM NGVTATRVYE RA
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus (By similarity).
Gene References into Functions
  1. Studied SNPs in cattle Fatty Acid Binding Protein 4 (FABP4) in several cattle breeds and evaluated possible associations with regards to meat quality and fat content of the meat. PMID: 28051918
  2. Overexpressed FABP4 of different genotypes in bovine intramuscular preadipocytes and studied the intracellular localization of FABP4 and the expression levels of lipid metabolism-related genes among different genotypes. PMID: 26913550
  3. These results provide an important basis for further understanding the regulation of bovine FABP4. PMID: 26641034
  4. This suggest that FABP4 affects milk yield and milk protein content, both economically important traits, and that further study of this gene is warranted. PMID: 26067182
  5. Data show that overexpression of cattle adipocyte fatty acid-binding protein (A-FABP)gene promoted fat deposition in the skeletal muscle of transgenic mice. PMID: 25867423
  6. FABP4 gene frequency and SNP genotypes in Holstein-Friesian cows and its relationship with protein and fat content in milk PMID: 25474885
  7. polymorphisms can be used to select for milk with lower concentrations of saturated fatty acids and higher concentrations of unsaturated fatty acids PMID: 23831098
  8. The effects of genetic polymorphisms of liver X receptor, alpha (LXR), stearoyl-CoA desaturase (SCD), Fatty acid synthase (FASN), and Fatty acid binding protein 4 (FABP4) were investigated on fatty acid composition in fat tissue of steers. PMID: 21615833
  9. The results confirm the lack of an association found by many other studies and suggest that these SNPs are not influential on the divergent intramuscular fat levels in the crossbred population tested. PMID: 20416823
  10. re-sequenced 4.3 kb of the FABP4 gene region in 24 Hanwoo bulls and identified 16 SNPs and 1 microsatellite polymorphism. PMID: 20331595
  11. The FABP4 gene falls into a suggestive/significant quantitative trait loci interval for beef marbling. PMID: 16879357
  12. Our findings suggest that the polymorphisms in FABP4 may play a role in determining one of the important genetic factors that influence back fat thickness in beef cattle. PMID: 18304447
  13. FABP4 I74V polymorphism had a significant effect on palmitoleic acid composition in intramuscular fat. PMID: 19077218
  14. A splice site single nucleotide polymorphism of the fatty acid binding protein 4 gene appears to be associated with intramuscular fat deposition in longissimus muscle in Australian cattle. PMID: 19466936

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Subcellular Location Cytoplasm, Nucleus
Protein Families Calycin superfamily, Fatty-acid binding protein (FABP) family
Database Links

KEGG: bta:281759

STRING: 9913.ENSBTAP00000000079

UniGene: Bt.97

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