Recombinant Bovine Hepatocyte growth factor (HGF)

Code CSB-YP758831BO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP758831BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP758831BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP758831BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HGF
Uniprot No.
Alternative Names
HGFHepatocyte growth factor; Hepatopoietin-A; Scatter factor; SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain]
Species
Bos taurus (Bovine)
Expression Region
32-494
Target Protein Sequence
QKKRRNTLH EFKRSAKTTL IKEDPLLKIK TKKMNTADQC ANRCIRNKGL PFTCKAFVFD KARKRCLWFP FNSMSSGVKK EFGHEFDLYE NKDYIRNCII GKGGSYKGTV SITKSGIKCQ PWNSMIPHEH SFLPSSYRGK DLQENYCRNP RGEEGGPWCF TSNPEVRYEV CDIPQCSEVE CMTCNGESYR GPMDHTETGK ICQRWDHQTP HRHKFLPERY PDKGFDDNYC RNPDGKPRPW CYTLDPDTPW EYCAIKMCAH STMNDTDLPM QTTECIQGQG EGYRGTINTI WNGIPCQRWD SQYPHQHDIT PENFKCKDLR ENYCRNPDGA ESPWCFTTDP NIRVGYCSQI PKCDVSSGQD CYRGNGKNYM GSLSKTRSGL TCSMWDKNME DLHRHIFWEP DATKLNKNYC RNPDDDAHGP WCYTGNPLIP WDYCPISRCE GDTTPTIVNL DHPVISCAKT KQLR
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
Gene References into Functions
  1. These results indicate that expression and production of HGF are regulated in a species-specific adipogenic differentiation-dependent manner and suggest that the decrease in HGF mRNA in mouse differentiated adipocytes is, at least in part, mediated by differentiation-dependent loss of its stability. PMID: 29040876
  2. Results confirm the prodevelopmental actions of activin A and indicate that CTGF may also function as an embryokine by regulating the number of ICM cells in the blastocyst and altering gene expression. Low concentrations of HGF were inhibitory to development. PMID: 27185102
  3. SNPs within bovine HGF gene were significantly associated with growth traits. This will provide a background for application of bovine HGF gene in the selection program in Chinese cattle. PMID: 23632198
  4. Treatment of the bovine satellite cells (BSC) with ephrin-A5 causes a reduction in velocity with a concomitant increase in directed migration. Treatment of BSC with hepatocyte growth factor had no immediate effect on cell motility or migration. PMID: 23296833
  5. HGF transiently increases gene transcription of angiotensin-converting enzyme PMID: 14679188

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Protein Families
Peptidase S1 family, Plasminogen subfamily
Database Links
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