Name: Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1)
Brand: CUSABIO
SKU: 018587BO

Product Details

Purity

>85% (SDS-PAGE)

Target Names

PPT1

Uniprot No.

P45478

Alternative Names

PPT1; CLN1; PPT; Palmitoyl-protein thioesterase 1; PPT-1; EC 3.1.2.22; Palmitoyl-protein hydrolase 1

Species

Bos taurus (Bovine)

Expression Region

28-306

Target Protein Sequence

DPP APLPLVIWHG MGDSCCNPLS MGAIKKMVEK KIPGIHVLSL EIGKTLREDV ENSFFLNVNS QVTTVCQILA KDPKLQQGYN AMGFSQGGQF LRAVAQRCPS PPMVNLISVG GQHQGVFGLP RCPGESSHIC DFIRKTLNAG AYNKAIQERL VQAEYWHDPI REDIYRNHSI FLADINQERG VNESYKKNLM ALKKFVMVKF LNDTIVDPVD SEWFGFYRSG QAKETIPLQE STLYTQDRLG LKAMDKAGQL VFLALEGDHL QLSEEWFYAH IIPFLE

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

Regarding your protein CSB-EP018587BO, could you please advise on the lead time, the sequencing, the pricing, and the expected tag information?
Also, have any customers reported this protein to be active?

Thanks for your inquiry.
Recombinant Bovine Palmitoyl-protein thioesterase 1(PPT1)
CSB-EP018587BO >> E.coli
Expression Region: 28-306aa; Full Length of Mature Protein
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

DPPAPLPLVIWHGMGDSCCNPLSMGAIKKMVEKKIPGIHVLSLEIGKTLREDVENSFFLNVNSQVTTVCQILAKDPKLQQGYNAMGFSQGGQFLRAVAQRCPSPPMVNLISVGGQHQGVFGLPRCPGESSHICDFIRKTLNAGAYNKAIQERLVQAEYWHDPIREDIYRNHSIFLADINQERGVNESYKKNLMALKKFVMVKFLNDTIVDPVDSEWFGFYRSGQAKETIPLQESTLYTQDRLGLKAMDKAGQLVFLALEGDHLQLSEEWFYAHIIPFLE

Sorry, as this is a semi-custom protein, which means we have never expressed any of these proteins before, so also haven't tested the activity or received the feedback from other customers, we can't 100% guarantee its activity.However, as what we provide is the full length of the mature protein, and it's purified under very mild condition, we recommend you to purchase a small size of eukaryotic expressed protein to have a try.
Generally, the more advanced expression system is the protein expressed with, the more likely to be active.
Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839016/
This literature introduces that the Human protein expressed by the Mammalian cell expression system is ative, while the homology between bovine and human protein is 94%. So in theory, the bovine protein should also be active.

Target Background

Function

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.

Subcellular Location

Lysosome. Secreted.

Protein Families

Palmitoyl-protein thioesterase family

Tissue Specificity

Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle.

Database Links

KEGG: bta:281421

STRING: 9913.ENSBTAP00000017780

UniGene: Bt.5019

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Code	CSB-YP018587BO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP018587BO-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP018587BO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP018587BO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1)

Product Details

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Target Background

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