Recombinant Bovine Rhodopsin kinase (GRK1), partial

Code CSB-YP009924BO
MSDS
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Source Yeast
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Code CSB-EP009924BO
MSDS
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Source E.coli
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Code CSB-EP009924BO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009924BO
MSDS
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Source Baculovirus
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Code CSB-MP009924BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GRK1
Uniprot No.
Alternative Names
GRK1; RHOK; Rhodopsin kinase GRK1; RK; EC 2.7.11.14; G protein-coupled receptor kinase 1
Species
Bos taurus (Bovine)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination. May play a role in the maintenance of the outer nuclear layer in the retina.
Gene References into Functions
  1. The C-terminal segment in GCAP2 confers target selectivity, facilitates membrane binding and provides sensitivity of the membrane localization of the protein to phosphorylation by rhodopsin kinase. PMID: 26001899
  2. The methylation status of GRK1 is affected by nucleotide binding and by the levels of free Ca2 + via recoverin. PMID: 22846544
  3. crystalline dimer interface was disrupted with a L166K mutation and the structure of GRK1-L166K was determined in complex with Mg(2+) . ATP to 2.5 A resolution PMID: 22684056
  4. A novel rhodopsin-kinase-binding site within the C-terminal region of recoverin. PMID: 21299498
  5. similar to transducin activation, rhodopsin phosphorylation by GRK1 and high affinity arrestin-1 binding only requires a rhodopsin monomer PMID: 20966068
  6. Recoverin and rhodopsin kinase have roles in a Ca2+-dependent feedback loop in membrane rafts from rod outer segments PMID: 15355976
  7. an interaction surface for the recoverin target rhodopsin kinase is constituted upon Ca2+ binding to the non-acylated mutant PMID: 15843174
  8. Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase PMID: 16675451
  9. Ca(2+)-bound recoverin is bound between rhodopsin and RK in a ternary complex on rod outer segment disk membranes, thereby blocking RK interaction with rhodopsin at high Ca(2+) PMID: 17020884
  10. key elements of rhodopsin kinase in different ligand states are involved in G protein-coupled receptor kinase activation PMID: 18339619

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Subcellular Location
Membrane; Lipid-anchor. Cell projection, cilium, photoreceptor outer segment.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily
Tissue Specificity
Rod outer segments of retina photoreceptor cells (at protein level). Retina.
Database Links

KEGG: bta:281457

STRING: 9913.ENSBTAP00000042484

UniGene: Bt.459

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