Your Good Partner in Biology Research
Your Good Partner in Biology Research
| Code | CSB-EP320768BJK |
| Size |
$2466Purchase it in Cusabio online store (only available for customers from the US) |
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| Description |
Intact bovine coronavirus (BCoV) nucleoprotein(N) cDNA (1-448AA) with an N-terminal 6xHis-tag was expressed in E.coli. The product is the recombinant full-length BCoV-N protein. The purity of this protein is greater than 85% determined by SDS-PAGE. Its predicted molecular weight is about 55.3 kDa. In addition to producing specific antibodies, this recombinant BCoV-N protein may be used in signal transduction studies. |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Target Names | N |
| Uniprot No. | P10527 |
| Research Area | Signal Transduction |
| Alternative Names | N; 7a; Nucleoprotein; Nucleocapsid protein; NC; Protein N |
| Species | Bovine coronavirus (strain Mebus) (BCoV)(BCV) |
| Source | E.coli |
| Expression Region | 1-448aa |
| Target Protein Sequence | MSFTPGKQSSSRASFGNRSGNGILKWADQSDQSRNVQTRGRRAQPKQTATSQLPSGGNVVPYYSWFSGITQFQKGKEFEFAEGQGVPIAPGVPATEAKGYWYRHNRRSFKTADGNQRQLLPRWYFYYLGTGPHAKDQYGTDIDGVFWVASNQADVNTPADILDRDPSSDEAIPTRFPPGTVLPQGYYIEGSGRSAPNSRSTSRASSRASSAGSRSRANSGNRTPTSGVTPDMADQIASLVLAKLGKDATKPQQVTKQTAKEIRQKILNKPRQKRSPNKQCTVQQCFGKRGPNQNFGGGEMLKLGTSDPQFPILAELAPTAGAFFFGSRLELAKVQNLSGNLDEPQKDVYELRYNGAIRFDSTLSGFETIMKVLNENLNAYQQQDGMMNMSPKPQRQRGQKNGQGENDNISVAAPKSRVQQNKSRELTAEDISLLKKMDEPYTEDTSEI Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 55.3 kDa |
| Protein Length | Full Length |
| Tag Info |
N-terminal 6xHis-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | Tris-based buffer，50% glycerol |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function (From Uniprot) |
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
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| Subcellular Location | Virion. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus. |
| Protein Families | Betacoronavirus nucleocapsid protein family |
