Recombinant Chicken Myosin light chain kinase, smooth muscle(Mylk),partial

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Code CSB-EP320904CHb3
Product Type Recombinant Protein
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Uniprot No. P11799
Relevance Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP320904CHb3 could indicate that this peptide derived from E.coli-expressed Gallus gallus (Chicken) Mylk.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP320904CHb3 could indicate that this peptide derived from E.coli-expressed Gallus gallus (Chicken) Mylk.
Storage Buffer Tris-based buffer,50% glycerol
Alias Telokin
Species Gallus gallus (Chicken)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence EVNYRTVTINTEQKVSDVYNIEERLGSGKFGQVFRLVEKKTGKVWAGKFFKAYSAKEKENIRDEISIMNCLHHPKLVQCVDAFEEKANIVMVLEMVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTSIKLIDFGLARRLESAGSLKVLFGTPEFVAPEVINYEPIGYETDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKSRLNCTQCLQHPWLQKDTKNMEAKKLSK
Research Area Signal Transduction
Source E.coli
Gene Names Mylk
Expression Region 1435-1722aa
Tag Info N-terminal 10xHis-SUMO-tagged and C-terminal MYC-tagged
Mol. Weight 52.9kDa
Protein Description Partial
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.
Subcellular Location Cytoplasm, cytosol, Membrane
Protein Families Protein kinase superfamily, CAMK Ser/Thr protein kinase family
Tissue Specificity Isoform telokin is expressed in gizzard, heart, lung, intestine, and skeletal muscle although the levels of the expression in the latter were much less than that in the gizzard.
Database Links

STRING: 9031.ENSGALP00000019112

UniGene: Gga.4091

Pathway Calcium signaling pathway
Focal adhesion
Regulation of actin cytoskeleton
Vascular smooth muscle contraction
Apelin signaling pathway
cGMP-PKG signaling pathway

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