Recombinant Clostridium botulinum Penicillin-binding protein 1A (pbpA),Partial

The recombinant Clostridium botulinum pbpA was expressed with the amino acid range of 663-830. The expected molecular weight for the pbpA protein is calculated to be 35.2 kDa. Expression of this pbpA protein is conducted in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the pbpA encoding gene fragment was conducted, allowing for easier detection and purification of the pbpA protein in subsequent stages.

Clostridium botulinum Penicillin-binding protein 1A (pbpA) is a crucial component involved in cell wall synthesis and maintenance within the bacterium Clostridium botulinum. Its primary function is to catalyze the formation of peptide cross-links in the bacterial cell wall, imparting structural integrity. In microbiology and antibacterial drug development, pbpA is a focal point for understanding antibiotic resistance mechanisms and exploring novel therapeutic targets. Its research areas encompass elucidating its role in cell wall biosynthesis and investigating strategies to combat antibiotic resistance in Clostridium botulinum. PbpA's significance lies in its contribution to fundamental bacterial biology and its potential as a target for antibacterial interventions.