Recombinant Danio rerio Deleted in azoospermia-like(dazl)

Code CSB-YP006514DIL
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Source Yeast
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Code CSB-EP006514DIL
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Source E.coli
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Code CSB-EP006514DIL-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006514DIL
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Source Baculovirus
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Code CSB-MP006514DIL
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names dazl
Uniprot No. Q9YGW7
Alternative Names dazl; zdazl; Deleted in azoospermia-like; DAZ-like protein; zDazl
Species Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region 1-229
Target Protein Sequence MVQGVQLPVC LICGLYSQDI QKHRQGFPSS LKLSNGYILP EGKMTPNTLF VGGIDMKVDE NEIREFFAKY GSVKEVKIIT YRGGICKGYG FVYFSEDVDI QTIVDQPISF KGKKLKLGPA IMKERSSRSV SSPMIGPSQW VNPTPYMYCS CCPPGLAPPS PVFSGGNQYM QPYSYSSPPG IMVPQVPMNY AQTTYAYQYP LPQWCGEQRT RLVNQNFVDC GVQTLLTLM
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function RNA-binding protein, which probably plays a central role in gametogenesis in both males and females. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and promoting the translation of key transcripts.
Gene References into Functions
  1. Cortical depth and differential transport of vegetally localized dorsal and germ line determinants, Wnt8a, grip2a, and Dazl, in the zebrafish embryo have been presented. PMID: 26528729
  2. that DAZL specifically binds the HuB 3'UTR and protects the message from degradation and/or enhances HuB translation, leading to the germ cell specific expression of HuB protein. PMID: 21695151
  3. DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly(A) tail length in zebrafish PMID: 19838299
  4. Data show that DAZ-like protein directly promotes the translation of the target mRNAs in vertebrates. PMID: 12296827
Subcellular Location Cytoplasm
Protein Families RRM DAZ family
Tissue Specificity Testis and ovary specific. In ovary, it is localized in the cortex of oocytes. At the onset of embryogenesis, maternal product is located at the vegetal pole, before migrating toward blastomeres through cytoplasmic streams as early embryogenesis proceeded
Database Links

KEGG: dre:58039

STRING: 7955.ENSDARP00000052602

UniGene: Dr.81259

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