Recombinant Danio rerio Deleted in azoospermia-like (dazl)

Code CSB-YP006514DIL
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Source Yeast
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Code CSB-EP006514DIL
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Source E.coli
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Code CSB-EP006514DIL-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006514DIL
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Source Baculovirus
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Code CSB-MP006514DIL
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dazl
Uniprot No.
Alternative Names
dazl; zdazl; Deleted in azoospermia-like; DAZ-like protein; zDazl
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-229
Target Protein Sequence
MVQGVQLPVC LICGLYSQDI QKHRQGFPSS LKLSNGYILP EGKMTPNTLF VGGIDMKVDE NEIREFFAKY GSVKEVKIIT YRGGICKGYG FVYFSEDVDI QTIVDQPISF KGKKLKLGPA IMKERSSRSV SSPMIGPSQW VNPTPYMYCS CCPPGLAPPS PVFSGGNQYM QPYSYSSPPG IMVPQVPMNY AQTTYAYQYP LPQWCGEQRT RLVNQNFVDC GVQTLLTLM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding protein involved in gametogenesis in both males and females. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and promoting the translation of key transcripts. Establishes oocyte polarity through interaction with Bucky ball (BUC). Interacts with Bucky ball (BUC) mRNA to mediate Balbiani body formation and oocyte polarity during early oogenesis.
Gene References into Functions
  1. Cortical depth and differential transport of vegetally localized dorsal and germ line determinants, Wnt8a, grip2a, and Dazl, in the zebrafish embryo have been presented. PMID: 26528729
  2. that DAZL specifically binds the HuB 3'UTR and protects the message from degradation and/or enhances HuB translation, leading to the germ cell specific expression of HuB protein. PMID: 21695151
  3. DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly(A) tail length in zebrafish PMID: 19838299
  4. Data show that DAZ-like protein directly promotes the translation of the target mRNAs in vertebrates. PMID: 12296827
Subcellular Location
Cytoplasm.
Protein Families
RRM DAZ family
Tissue Specificity
Testis and ovary specific. In ovary, it is localized in the cortex of oocytes. At the onset of embryogenesis, maternal product is located at the vegetal pole, before migrating toward blastomeres through cytoplasmic streams as early embryogenesis proceeded
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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