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Recombinant Danio rerio Glial fibrillary acidic protein (gfap)

Recombinant Danio rerio Glial fibrillary acidic protein (gfap)

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Code	CSB-EP009369DIL
Abbreviation	Recombinant Zebrafish gfap protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

gfap

Uniprot No.

Q58EE9

Research Area

Neuroscience

Alternative Names

gfapGlial fibrillary acidic protein; GFAP

Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Source

E.coli

Expression Region

1-444aa

Target Protein Sequence

MESQRSFSSYRKRFGTPGGSPSVGVTSRHSTGRLSLHSSPRHLTSSPLTLSTSRLSLGGERLDFSADSLLKAQYRETRTNEKVEMMGLNDRFASYIEKVRFLEQQNKMLVAELNQLRGKEPSRLGDIYQEELRELRRQVDGLNAGKARLEIERDNLASDLGTLKQRLQDETALRQEAENNLNTFRQDVDEAALNRVQLERKIEALQDEINFLKKVHEEEMRELHEQLMAQQVHVDLDVSKPDLTAALKEIRAQFEAMANSNMQETEEWYRSKFADLTDAANRNGEALRQAKQEANDYRRQIQGLTCDLESLRGSNESLERQLREMEERFAIETAGYQDTVARLEDEIQMLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEESRITVPVQNFTNLQFRDTSMDTKLTPEAHVKRSIVVRTVETRDGEIIKESTTERKDLP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

71.2kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The gene responsible for the Danio rerio (Zebrafish) gfap protein (1-444aa) is incorporated into a plasmid vector, forming recombinant plasmid. The resulting recombinant plasmid is introduced into e.coli cells, from which cells survive in the presence of a specific antibiotic are selected. The selected e.coli cells containing the recombinant plasmid are cultured under conditions that facilitate the expression of the gene of interest. A N-terminal 10xHis-SUMO tag and C-terminal Myc tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Danio rerio (Zebrafish) gfap protein from the cell lysate. Denaturing SDS-PAGE is employed to resolve the resulting recombinant Danio rerio (Zebrafish) gfap protein, revealing a purity greater than 85%.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

Aggregation-prone GFAP mutation in Alexander disease was validated using a zebrafish model; The p.Asp128Asn GFAP mutation is likely to be a disease-causing mutation PMID: 28882119
The distribution of GFAP immunoreactivity implies that enteric glia are widespread in the fish gastrointestinal tract. PMID: 20512593
Generation of transgenic zebrafish that express green fluorescent protein (GFP) in glial cells driven by the zebrafish glial fibrillary acidic protein (GFAP) regulatory elements. PMID: 16765104
Cells expressing the two reporters display radial glial morphology, colocalize with the NSC marker Sox2, undergo proliferation, and are capable of self-renewal within the matrix of distinct thickness in the telencephalon. PMID: 19161226

Subcellular Location

Cytoplasm, cytoskeleton. Note=Associated with intermediate filaments.

Protein Families

Intermediate filament family

Database Links

KEGG: dre:30646

STRING: 7955.ENSDARP00000029607

UniGene: Dr.32667

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