Recombinant Danio rerio Glial fibrillary acidic protein (gfap)

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Code CSB-EP009369DIL
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
gfap
Uniprot No.
Research Area
Neuroscience
Alternative Names
gfapGlial fibrillary acidic protein; GFAP
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Source
E.coli
Expression Region
1-444aa
Target Protein Sequence
MESQRSFSSYRKRFGTPGGSPSVGVTSRHSTGRLSLHSSPRHLTSSPLTLSTSRLSLGGERLDFSADSLLKAQYRETRTNEKVEMMGLNDRFASYIEKVRFLEQQNKMLVAELNQLRGKEPSRLGDIYQEELRELRRQVDGLNAGKARLEIERDNLASDLGTLKQRLQDETALRQEAENNLNTFRQDVDEAALNRVQLERKIEALQDEINFLKKVHEEEMRELHEQLMAQQVHVDLDVSKPDLTAALKEIRAQFEAMANSNMQETEEWYRSKFADLTDAANRNGEALRQAKQEANDYRRQIQGLTCDLESLRGSNESLERQLREMEERFAIETAGYQDTVARLEDEIQMLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEESRITVPVQNFTNLQFRDTSMDTKLTPEAHVKRSIVVRTVETRDGEIIKESTTERKDLP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
71.2kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The gene responsible for the Danio rerio (Zebrafish) gfap protein (1-444aa) is incorporated into a plasmid vector, forming recombinant plasmid. The resulting recombinant plasmid is introduced into e.coli cells, from which cells survive in the presence of a specific antibiotic are selected. The selected e.coli cells containing the recombinant plasmid are cultured under conditions that facilitate the expression of the gene of interest. A N-terminal 10xHis-SUMO tag and C-terminal Myc tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Danio rerio (Zebrafish) gfap protein from the cell lysate. Denaturing SDS-PAGE is employed to resolve the resulting recombinant Danio rerio (Zebrafish) gfap protein, revealing a purity greater than 85%.

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Target Background

Gene References into Functions
  1. Aggregation-prone GFAP mutation in Alexander disease was validated using a zebrafish model; The p.Asp128Asn GFAP mutation is likely to be a disease-causing mutation PMID: 28882119
  2. The distribution of GFAP immunoreactivity implies that enteric glia are widespread in the fish gastrointestinal tract. PMID: 20512593
  3. Generation of transgenic zebrafish that express green fluorescent protein (GFP) in glial cells driven by the zebrafish glial fibrillary acidic protein (GFAP) regulatory elements. PMID: 16765104
  4. Cells expressing the two reporters display radial glial morphology, colocalize with the NSC marker Sox2, undergo proliferation, and are capable of self-renewal within the matrix of distinct thickness in the telencephalon. PMID: 19161226
Subcellular Location
Cytoplasm, cytoskeleton. Note=Associated with intermediate filaments.
Protein Families
Intermediate filament family
Database Links
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