Recombinant Debaryomyces hansenii Autophagy protein 5(ATG5)

Code CSB-YP728218DIS
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Source Yeast
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Code CSB-EP728218DIS
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Source E.coli
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Code CSB-EP728218DIS-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP728218DIS
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Source Baculovirus
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Code CSB-MP728218DIS
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ATG5
Uniprot No. Q6BVI8
Alternative Names ATG5; DEHA2C02332g; Autophagy protein 5
Species Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Expression Region 1-292
Target Protein Sequence MQSSNELIEI KDKLWNGSIN VRILMGDDNI KDPKEFLITV YRNSYFPIYF PSVITYFQKY NEKIKYMPVW LEYETVPIKW NLPIGVLYDL LHLSSIVQNR EDSSWTLTLR FSDDYPTDQV IPFTYTDVDN SVNYNKSLKE VVVNQLKQSC FVINGNSKPI MNLSEKDSDE LWNSIRIHNL KSFNQINKKI IPIQKKFQKL PVKIYIPGSA TIIHAPIYPY SDSGEAVLLR DILEEYLPDL MSSNNETLGS IYIHGINVET IINKDIIDVW ELFKHLDNFL YIIVLFSTYS TI
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy). Also required for mitophagy, which eliminates defective or superfluous mitochondria in order to fulfill cellular energy requirements and prevent excess ROS production. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 and ATG8 association to the vesicle membranes.
Subcellular Location Preautophagosomal structure membrane; Peripheral membrane protein.
Protein Families ATG5 family
Database Links

KEGG: dha:DEHA2C02332g

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