Recombinant Dictyostelium discoideum Ras-related protein Rab-11A (rab11A)

Code CSB-YP019153DKK
MSDS
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Source Yeast
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Code CSB-EP019153DKK
MSDS
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Source E.coli
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Code CSB-EP019153DKK-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019153DKK
MSDS
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Source Baculovirus
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Code CSB-MP019153DKK
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rab11A
Uniprot No.
Alternative Names
rab11A; rab11; DDB_G0269238; Ras-related protein Rab-11A
Species
Dictyostelium discoideum (Slime mold)
Expression Region
1-214
Target Protein Sequence
MTSKGSQEEY DYLYKIVLIG DSGVGKSNLL SRFTRNEFSL ETKSTIGVEF ATRTIQTEGK TIKAQVWDTA GQERYRAITS AYYRGAVGAL LVYDIAKQAT YKSVERWILE LRENADRNIE IMLVGNKSDL RHLREVSTDE AKEFSEKHKL TFIETSALDS SNVELAFQNI LTQIYHIMSR PSHSTGPQTT IDSNTETIIL PTTSEPPAAK SGCC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for normal contractile vacuole structure and function. Cells expressing a dominant negative rab11A exhibit a more extensive contractile vacuole network and enlarged contractile vacuole bladders. These cells exhibit a functional defect in osmotic regulation where cells immersed in water become rounded and detach from the surface, and contain swollen contractile vacuoles.
Gene References into Functions
  1. These findings suggest that P2XA activation results in vacuolar calcium release, which triggers activation of CnrF Rab gtpase activating protein activity and subsequent downregulation of Rab11a to allow vacuole fusion. PMID: 24335649
Subcellular Location
Contractile vacuole membrane; Lipid-anchor; Cytoplasmic side.
Protein Families
Small GTPase superfamily, Rab family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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