Recombinant Drosophila melanogaster Dosage compensation regulator (mle), partial

Code CSB-YP333021DLU
MSDS
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Source Yeast
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Code CSB-EP333021DLU
MSDS
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Source E.coli
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Code CSB-EP333021DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333021DLU
MSDS
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Source Baculovirus
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Code CSB-MP333021DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mle
Uniprot No.
Alternative Names
mle; nap; CG11680; Dosage compensation regulator; EC 3.6.4.13; ATP-dependent RNA helicase mle; Protein male-less; Protein maleless; Protein no action potential
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required in males for dosage compensation of X chromosome linked genes. Mle, msl-1 and msl-3 are colocalized on X chromosome. Each of the msl proteins requires all the other msls for wild-type X-chromosome binding. Probably unwinds double-stranded DNA and RNA in a 3' to 5' direction.
Gene References into Functions
  1. We have employed a systems biology approach (microarray) to investigate the global aneuploid effect of the maleless (mle) mutation that disrupts the binding of male specific lethal (MSL) proteins that function in dosage compensation. PMID: 27456781
  2. MLE specifically targets hairpin RNAs at their site of transcription. The association of MLE at these sites is independent of sequence and chromosome location PMID: 26752049
  3. The Drosophila Helicase MLE is Implicated in Functions Distinct From its Role in Dosage Compensation PMID: 25776889
  4. Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling. PMID: 26545078
  5. UNR facilitates the interaction of MLE with the lncRNA roX2 during Drosophila dosage compensation. PMID: 25158899
  6. Study reports that mle (napts) mutant flies exhibit developmental lethality, decreased fecundity and increased neurodegeneration. PMID: 22513411
  7. Data suggest that MLE can stimulate the transcription activity of rox2 and that MLE associates with rox2 through direct interaction with a newly identified 54-bp repeat, Prox. PMID: 15358781
  8. Zn72D is required for productive splicing of the transcript for the MSL protein Maleless, explaining the dosage compensation defect. PMID: 17923683
  9. the ATPase activity is sufficient for MLE's role in transcriptional enhancement, while the helicase activity is necessary for the spreading of the complex along the X chromosome PMID: 18039854
  10. A maleless structure-function analysis clarified the domain requirements for RNA interaction, helicase activity and localization to the X chromosomal territory. PMID: 18086708
  11. the minimal transactivation domain of MLE mediates the expression of MLE target genes through recruitment of Pol II PMID: 18360693

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Subcellular Location
Nucleus. Chromosome. Note=Mle is associated with hundreds of discrete sites along the length of the X chromosome in males and not in females, and is associated with 30-40 autosomal sites in both sexes.
Protein Families
DEAD box helicase family, DEAH subfamily
Database Links

KEGG: dme:Dmel_CG11680

STRING: 7227.FBpp0085367

UniGene: Dm.2901

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