Recombinant Drosophila melanogaster Maternal protein pumilio (pum), partial

Code CSB-EP326575DLU
MSDS
Size $256
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Target Names
pum
Uniprot No.
Research Area
Others
Species
Drosophila melanogaster (Fruit fly)
Source
E.coli
Expression Region
1091-1330aa
Target Protein Sequence
GRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
31.6
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of deadenylase complexes leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Mediates post-transcriptional silencing of E2f mRNA by binding to its 3'-UTR and promoting miRNA regulation. Required for abdominal development and to support proliferation and self-renewal of germ cells. Pum is the only gene required for nos activity that is not also required for posterior localization of germline determinants. Pum is required during embryogenesis when nos activity apparently moves anteriorly from the posterior pole.
Gene References into Functions
  1. The specificity of a complex between Drosophila RNA-binding protein Pumilio and its target RNA of Nanos has been described. PMID: 27729296
  2. Crystal structures of Nanos-Pumilio-RNA complexes reveal that Nanos embraces Pumilio and RNA, contributes sequence-specific contacts, and increases Pumilio RNA-binding affinity. PMID: 27482653
  3. reductions in the expression of the Pumilio translational repressor complex enhanced phenotypes due to dLsd1 depletion in Drosophila. the PUM complex is a target of LSD1 regulation. PMID: 26438601
  4. Pumilio has a novel mRNA decay function during neural development. Decreased expression stabilized predicted neural mRNA targets. A Pumilio recognition element is needed to trigger reporter-transcript decay in the nervous system. PMID: 25896902
  5. Nos and Pum are required to maintain the dendritic complexity of da neurons during larval growth by promoting the outgrowth of new dendritic branches and the stabilization of existing dendritic branches PMID: 22391052
  6. Pumilio complexes repress dE2F1 levels PMID: 22345517
  7. Data show that the extended 3' UTR sequences are selectively expressed in neural tissues and contain putative recognition motifs for the translational repressor, Pumilio, which also exhibits the 3' lengthening phenomenon. PMID: 21896737
  8. The studies suggest that, in cystoblasts, Bam and Bgcn may directly inhibit Pum/Nos activity to promote differentiation of germ line stem cells. PMID: 20018853
  9. affects anterior determination in drosophila embryo; temporally regulates bicoid (bcd) mRNA translation via evolutionarily conserved Nanos response elements (NRE) in its 3'UTR PMID: 12015297
  10. a translaitonal repressor that regulates neuronal excitability PMID: 12136020
  11. Pumilio represses translation of differentiation-promoting mRNAs in germline stem cells. PMID: 15668176
  12. A characteristic sequence motif in the 3'-untranslated regions of mRNAs associated with PUMILIO was identified. PMID: 16537387
  13. Regulatory interplay between Nanos and Pum could cause a small change in Pum activity to be amplified into a large shift in the balance between GluRIIA and GluRIIB synapses. PMID: 19403823

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Subcellular Location
Cytoplasm. Cytoplasm, Cytoplasmic ribonucleoprotein granule.
Database Links

KEGG: dme:Dmel_CG9755

STRING: 7227.FBpp0305823

UniGene: Dm.1115

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