Product Details

Uniprot No.

P25822

Target Names

pum

Alternative Names

Maternal protein pumilio pum CG9755

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) pum protein (1091-1330aa)

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

In the generation of the pum polyclonal antibody, a rabbit is immunized with a recombinant Drosophila melanogaster (Fruit fly) pum protein segment (1091-1330aa) to trigger an antibody response. The rabbit serum is obtained and then purified using affinity chromatography to harvest polyclonal antibodies against pum. The pum antibody is recommended for detecting the Drosophila melanogaster (Fruit fly) pum protein through ELISA and WB assays.

The Pumilio (Pum) protein in Drosophila melanogaster is a crucial regulator of post-transcriptional gene expression. Acting as an RNA-binding protein, Pumilio binds to specific sequence motifs in the 3' untranslated regions of target mRNAs, leading to the repression of translation and mRNA degradation. Pumilio plays pivotal roles in various biological processes, including germline development, where it regulates the expression of genes involved in germ cell fate and differentiation. In embryonic development, Pumilio is essential for establishing polarity and proper patterning by controlling the translation of key mRNAs.

Target Background

Function

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of deadenylase complexes leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Mediates post-transcriptional silencing of E2f mRNA by binding to its 3'-UTR and promoting miRNA regulation. Required for abdominal development and to support proliferation and self-renewal of germ cells. Pum is the only gene required for nos activity that is not also required for posterior localization of germline determinants. Pum is required during embryogenesis when nos activity apparently moves anteriorly from the posterior pole.

Gene References into Functions

The specificity of a complex between Drosophila RNA-binding protein Pumilio and its target RNA of Nanos has been described. PMID: 27729296
Crystal structures of Nanos-Pumilio-RNA complexes reveal that Nanos embraces Pumilio and RNA, contributes sequence-specific contacts, and increases Pumilio RNA-binding affinity. PMID: 27482653
reductions in the expression of the Pumilio translational repressor complex enhanced phenotypes due to dLsd1 depletion in Drosophila. the PUM complex is a target of LSD1 regulation. PMID: 26438601
Pumilio has a novel mRNA decay function during neural development. Decreased expression stabilized predicted neural mRNA targets. A Pumilio recognition element is needed to trigger reporter-transcript decay in the nervous system. PMID: 25896902
Nos and Pum are required to maintain the dendritic complexity of da neurons during larval growth by promoting the outgrowth of new dendritic branches and the stabilization of existing dendritic branches PMID: 22391052
Pumilio complexes repress dE2F1 levels PMID: 22345517
Data show that the extended 3' UTR sequences are selectively expressed in neural tissues and contain putative recognition motifs for the translational repressor, Pumilio, which also exhibits the 3' lengthening phenomenon. PMID: 21896737
The studies suggest that, in cystoblasts, Bam and Bgcn may directly inhibit Pum/Nos activity to promote differentiation of germ line stem cells. PMID: 20018853
affects anterior determination in drosophila embryo; temporally regulates bicoid (bcd) mRNA translation via evolutionarily conserved Nanos response elements (NRE) in its 3'UTR PMID: 12015297
a translaitonal repressor that regulates neuronal excitability PMID: 12136020
Pumilio represses translation of differentiation-promoting mRNAs in germline stem cells. PMID: 15668176
A characteristic sequence motif in the 3'-untranslated regions of mRNAs associated with PUMILIO was identified. PMID: 16537387
Regulatory interplay between Nanos and Pum could cause a small change in Pum activity to be amplified into a large shift in the balance between GluRIIA and GluRIIB synapses. PMID: 19403823

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Subcellular Location

Cytoplasm. Cytoplasm, Cytoplasmic ribonucleoprotein granule.

Database Links

KEGG: dme:Dmel_CG9755

STRING: 7227.FBpp0305823

UniGene: Dm.1115

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Code	CSB-PA326575XA01DLU
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: pum Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
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pum Antibody

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