Recombinant Drosophila melanogaster Protein Vhl (Vhl)

Code CSB-YP893700DLU
MSDS
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Source Yeast
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Code CSB-EP893700DLU
MSDS
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Source E.coli
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Code CSB-EP893700DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893700DLU
MSDS
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Source Baculovirus
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Code CSB-MP893700DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Vhl; CG13221Protein Vhl
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-178
Target Protein Sequence
MALQIAQNNR DGQQLVGADQ GKVEVYVLFA NTTYRTLDLY WVCERERENM YLTLKPFEEV RVNTFTTHSW LFRDYYTGER MHVRSQRIFQ PIRVRVPKSQ QSPDQLVDVR SEVLIHFPMR SLRENCLWLV ARWLIRTSNA PRRIIHGYHI PSTLKQQLLS LLTCIESYSR VAGTRRRR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in development of tracheal vasculature. Probably involved in halting cell migration at the end of vascular tube outgrowth. Possesses E3 ubiquitin ligase activity when in complex with Elongin BC complex, Cul2 and Rox1a/Rbx1, and can target sima/Hif1a for ubiquitination. May play a critical role in promoting microtubule stabilization when tubulins are correctly folded by the prefoldin complex. If tubulin is incorrectly folded, may promote its degradation.
Gene References into Functions
  1. Transgenic pVHL can fully maintain normal dVHL-HIF1-alpha downstream pathways in flies. PMID: 25310726
  2. The results establish a developmental function of the VHL gene that is relevant to its tumor-suppressor activity. PMID: 20388653
  3. findings reveal a second type of tracheal hypoxic response in which Sima activation conflicts with developmental tracheogenesis, and identify the dVHL and ago ubiquitin ligases as key determinants of hypoxia sensitivity in tracheal cells PMID: 19285057
Protein Families
VHL family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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