Recombinant Drosophila melanogaster Ras-like protein 1 (Ras85D)

Code CSB-YP362473DLU
MSDS
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Source Yeast
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Code CSB-EP362473DLU
MSDS
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Source E.coli
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Code CSB-EP362473DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP362473DLU
MSDS
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Source Baculovirus
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Code CSB-MP362473DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ras85D
Uniprot No.
Alternative Names
Ras85D; Ras1; CG9375; Ras-like protein 1; Dras1; Dmras85D
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-186
Target Protein Sequence
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLL VFAVNSAKSF EDIGTYREQI KRVKDAEEVP MVLVGNKCDL ASWNVNNEQA REVAKQYGIP YIETSAKTRM GVDDAFYTLV REIRKDKDNK GRRGRKMNKP NRRFKC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays a role in eye development by regulating cell growth, survival of postmitotic ommatidial cells and differentiation of photoreceptor cells. During larval development, mediates Ptth/tor signaling leading to the production of ecdysone, a hormone required for the initiation of metamorphosis.
Gene References into Functions
  1. In conclusion, Ras(V12)-modified caspases initiate a feedback amplification loop involving tumorous epithelial cells and macrophage-like immune cells that is necessary for uncontrolled tumor growth and invasive behavior. PMID: 28853394
  2. Thus, our current work provides a novel mechanism of pupal size determination by microRNAs during Drosophila melanogaster metamorphosis. PMID: 27733364
  3. Oncogenic Ras stimulates Eiger/TNF exocytosis to promote growth. PMID: 25411211
  4. Inhibition of Ras1 signaling leads to reduced Appl expression, whereas constitutive activation drives ectopic Appl expression. PMID: 23178937
  5. Data show that the ras1(R68Q) mutant is homozygous viable but causes various developmental defects associated with elevated Ras signaling. PMID: 21858158
  6. Results conclude that the membrane association of Drosophila Ras1 is not required for eye development. PMID: 20937772
  7. interaction between Ras(V12) and scrib(-) clones involves JNK signalling propagation and JNK-induced upregulation of JAK/STAT-activating cytokines, a compensatory growth mechanism for tissue homeostasis PMID: 20072127
  8. In the Drosophila eye, Ras may protect cells from apoptosis by inhibiting both JNK and Hid activities. Surprisingly, reducing Ras activity in the wing, however, does not cause apoptosis but rather affects cell and organ size. PMID: 19840402
  9. Ras activity in the prothoracic gland regulates body size and the duration of each larval stage by regulating ecdysone release. PMID: 16182526

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Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Loss of prenylation causes protein location to the cytoplasm.
Protein Families
Small GTPase superfamily, Ras family
Tissue Specificity
Expressed in the posterior termini of the embryo, restricted mainly to the embryonic central nervous system, and in the eye imaginal disk.
Database Links

KEGG: dme:Dmel_CG9375

STRING: 7227.FBpp0081600

UniGene: Dm.4812

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