Recombinant Drosophila melanogaster Transmembrane GTPase Marf(Marf) ,partial

Code CSB-YP801787DLU
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Source Yeast
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Code CSB-EP801787DLU
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Source E.coli
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Code CSB-EP801787DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP801787DLU
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Source Baculovirus
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Code CSB-MP801787DLU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Marf
Uniprot No. Q7YU24
Alternative Names Marf; dmfn; CG3869Transmembrane GTPase Marf; EC 3.6.5.-; Mitochondrial assembly regulatory factor; Mitofusin
Species Drosophila melanogaster (Fruit fly)
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission (By similarity).
Gene References into Functions
  1. Enhancing the profusion gene mitofusin/marf is beneficial in an in vivo model of TDP-43 proteinopathies, serving as a potential therapeutic target. PMID: 28324764
  2. activation of endoplasmic reticulum stress by defective mitochondria is neurotoxic in pink1 and parkin flies and that the reduction of this signalling is neuroprotective, independently of defective mitochondria. PMID: 27336715
  3. Clu is upstream of and binds to VCP in vivo and promotes VCP-dependent Marf degradation in vitro Marf accumulates in whole muscle lysates of clu-deficient flies and is destabilized upon Clu overexpression. Thus, Clu is essential for mitochondrial homeostasis and functions in concert with Parkin and VCP for Marf degradation to promote damaged mitochondrial clearance. PMID: 26931463
  4. lack of ChChd3 leads to inactivation of Hippo activity under normal development, which is also dependent on the transcriptional coactivator Yorkie (Yki). Furthermore, loss of ChChd3 induces oxidative stress and activates the JNK pathway. In addition, depletion of other mitochondrial fusion components, Opa1 or Marf, inactivates the Hippo pathway as well. PMID: 27317679
  5. Marf is required for mitochondrial fusion and transport in long axons. PMID: 25313867
  6. Expression of Mfn2 and endoplasmic reticulum (ER) stress reduction in flies lacking Marf corrected ER shape, attenuating the developmental and motor defects. PMID: 24469638
  7. Parkin deficiency and resulting mitophagic disruption produces cardiomyopathy which can be contained by suppressing mitofusin. PMID: 24192653
  8. mfn2 mutations alter mitochondrial dynamics and induce retinal and cardiac pathology PMID: 22957060
  9. Data report here that Drosophila Reaper can induce mitochondrial fragmentation by binding to and inhibiting the pro-fusion protein MFN2 and its Drosophila counterpart dMFN/Marf. PMID: 21475305
  10. MARF and Opa1 control mitochondrial and cardiac function in Drosophila. PMID: 21148429
  11. The PINK1/Parkin pathway affects mitochondrial fission/fusion as suggested by previous genetic interaction studies. PMID: 20194754
  12. Dmfn-mRNA was widely expressed during embryogenesis accumulating in the mesoderm and endoderm during gut development, during oogenesis with transcripts maternally deposited into the early embryo and in the male germ line. PMID: 12128227

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Subcellular Location Mitochondrion outer membrane, Multi-pass membrane protein
Protein Families TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, Mitofusin subfamily
Tissue Specificity Widely expressed in embryos, accumulating in the mesoderm and endoderm during gut development. In the male germ line, it is expressed in spermatogonia, spermatocytes and early spermatids.
Database Links

KEGG: dme:Dmel_CG3869

STRING: 7227.FBpp0070873

UniGene: Dm.11038

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