Recombinant Drosophila melanogaster cAMP-dependent protein kinase catalytic subunit (Pka-C1)

Code CSB-YP318954DLU
MSDS
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Source Yeast
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Code CSB-EP318954DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP318954DLU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP318954DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pka-C1
Uniprot No.
Alternative Names
Pka-C1; CdkA; DC0; CG4379; cAMP-dependent protein kinase catalytic subunit 1; PKA C; EC 2.7.11.11; Protein kinase DC0
Species
Drosophila melanogaster (Fruit fly)
Expression Region
2-353
Target Protein Sequence
GNNATTSNK KVDAAETVKE FLEQAKEEFE DKWRRNPTNT AALDDFERIK TLGTGSFGRV MIVQHKPTKD YYAMKILDKQ KVVKLKQVEH TLNEKRILQA IQFPFLVSLR YHFKDNSNLY MVLEYVPGGE MFSHLRKVGR FSEPHSRFYA AQIVLAFEYL HYLDLIYRDL KPENLLIDSQ GYLKVTDFGF AKRVKGRTWT LCGTPEYLAP EIILSKGYNK AVDWWALGVL VYEMAAGYPP FFADQPIQIY EKIVSGKVRF PSHFGSDLKD LLRNLLQVDL TKRYGNLKAG VNDIKNQKWF ASTDWIAIFQ KKIEAPFIPR CKGPGDTSNF DDYEEEALRI SSTEKCAKEF AEF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Serine/threonine-protein kinase involved in memory formation. Promotes long-term memory by phosphorylating meng and by regulating CrebB protein stability and activity. As part of ethanol response in the glia, mediates ethanol-induced structural remodeling of actin cytoskeleton and perineurial membrane topology when anchored to the membrane.
Gene References into Functions
  1. Hedgehog signalling activation causes PKA to switch its substrates from Ci to Smo within the Hh signalling complex. PMID: 25289679
  2. tightly controlled dose-dependent intra-neuronal PKA activity levels are critical in determining the dendritic arbor complexity, one of the possible ways being through the regulation of organelle distribution. PMID: 25017992
  3. Alpha- NAC is a substrate of PKA following Parathyroid Hormone signaling. PMID: 24550008
  4. that manipulations that increase cAMP/PKA signaling in adipokinetic hormone (AKH)-producing cells at the base of the neuroendocrine ring gland restore the dNf1 growth deficiency. PMID: 24278035
  5. Acute expression of protein kinase A inhibitory peptide in aged animals is sufficient to reverse age-related memory impairment in wild-type animals. PMID: 21084612
  6. Two-dimensional gel electrophoresis, (32)P labelling and immunodetection show that "in vitro" PKA phosphorylates the serine in the C-terminus of the NDUFS4 subunit in isolated bovine complex I. PMID: 20433953
  7. Its signaling underlies age-related memory impairment.(review) PMID: 18646611
  8. We found that decreased PKA activity in neurons rendered flies insensitive to the wake-promoting effects of octopamine. PMID: 18799671

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Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily
Tissue Specificity
More abundant in adult head than adult body.
Database Links

KEGG: dme:Dmel_CG4379

STRING: 7227.FBpp0079448

UniGene: Dm.2136

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