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Recombinant Escherichia phage P1 Recombinase cre (cre)

In Stock
Code CSB-EP362000EDD
Abbreviation Recombinant Escherichia phage P1 Recombinase cre protein
MSDS
MSDS Datasheet
Size US$388
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  • CSB-EP362000EDD SDS-page
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
cre
Uniprot No.
P06956
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
cre; Recombinase cre
Species
Escherichia phage P1 (Bacteriophage P1)
Source
E.coli
Expression Region
1-343aa
Target Protein Sequence
MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDGD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
46.0 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Protein FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The expression of the recombinant Escherichia phage P1 (Bacteriophage P1) cre protein involves the construction of a plasmid encoding the Escherichia phage P1 (Bacteriophage P1) cre protein (1-343aa). This resulting plasmid is introduced into e.coli cells. Positive e.coli cells are selected and cultured for the protein expression. The protein is fused with a N-terminal 10xHis tag and C-terminal Myc tag. After that, cultured cells are lysed. The subsequent step includes purifying the recombinant Escherichia phage P1 (Bacteriophage P1) cre protein through affinity purification from the cell lysate and assessing the purity of the protein via SDS-PAGE. The purity of the recombinant cre protein is greater than 85%.

Related Products

cre Antibodies

cre Proteins

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Target Background

Function
Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
Gene References into Functions
  1. This study demonstrates that Col6a1-Cre driver mice are as useful as Twist2-Cre driver mice for functional analyses of GALT-resident mesenchymal cells, including MCi cells. PMID: 28882590
  2. Cre-recombinase in aP2-Cre mice, showed strong Cre activity in the dorsal hindbrain region and vertebrae of E12.5 embryos. PMID: 22150821
  3. Cre-mediated synapsis of loxP sites occurs with a high affinity and is pH-dependent; the crystal structures of a pre-cleavage Cre-loxP synaptic complex in a configuration representing the starting point in the recombination pathway were determined PMID: 17573343
  4. The GnRH-CRETeR mouse can be used to study genes that may have distinct roles in reproductive physiology during the various developmental stages. PMID: 18445125
Protein Families
'phage' integrase family
Database Links

KEGG: vg:2777477

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