Recombinant Enterobacteria phage T4 Recombination protein uvsY (uvsY)

Intact Enterobacteria phage T4 Recombination protein uvsY (uvsY) cDNA (1-137aa) with an N-terminal 6xHis--SUMO-tag was expressed in the E.coli. The forming protein is the Recombinant full-length Enterobacteria phage T4 uvsY protein. The purity of this protein is greater than 90% as determined by SDS-PAGE. Under reducing conditions, the SDS-PAGE gel showed a molecular weight band of about 33 kDa. And this uvsY protein also underwent the validation by the LC-MS/MS analysis. This uvsY protein is in-stock so that there is no waiting period for product preparation. It may find uses for specific antibody synthesis or in the studies of homologous recombination (HR).

UVSY is the phage T4 recombination mediator protein (RMP) that is crucial for efficient HR in bacteriophage T4 and is the functional analog of the eukaryotic Rad52 protein. HR has important roles in repairing double-stranded DNA (dsDNA) break repair, restarting the stalled replication forks, and recombination-dependent replication. UVSY is specifically recruited to ssDNA–Gp32 complexes and promotes the dissociation of Gp32, thus stimulating the DNA-dependent ATPase activity of recombinase UVSX and facilitating the strand exchange.