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Recombinant Enterobacteria phage T4 Endonuclease II (denA)

Recombinant Enterobacteria phage T4 Endonuclease II (denA)

Code	CSB-YP357009EDZ
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP357009EDZ
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP357009EDZ-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP357009EDZ
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP357009EDZ
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

denA

Uniprot No.

P07059

Alternative Names

denA; Endonuclease II; EC 3.1.21.8

Species

Enterobacteria phage T4 (Bacteriophage T4)

Expression Region

1-144

Target Protein Sequence

MGRKAHFIMK EIATEYSFIK YTELELDDNG SIKQLSIPNK YNVIYAIAIN DELVYIGKTK NLRKRINYYR TAINRKDKTS DSTKSALIHS ALKEGSKVEF YARQCFNLSM TNELGTMTIA TIDLEEPLFI KLFNPPWNIQ HKKK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Contributes to the degradation of host DNA, permitting the scavenging of host-derived nucleotides for phage DNA synthesis. Sequence-specific endonuclease. Catalyzes nicking of the bottom strand of double-stranded DNA between the first and second base pair to the right of a top-strand CCGC motif. Does not cleave native phage DNA, which contains 5-hydroxymethylcytosine instead of cytosine.

Gene References into Functions

EndoII forms a striking X-shaped tetrameric structure composed as a dimer of dimers, with a protruding hairpin domain not present in UvrC or I-TevI providing most of the dimerization and tetramerization interfaces. PMID: 20156453
The conserved glycine (G49) and arginine (R57) were essential for normal sequence recognition. PMID: 18539732

Database Links

KEGG: vg:1258710

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