Name: Recombinant Escherichia coli Antitoxin RelB (relB)
Brand: CUSABIO
SKU: CSB-EP314742ENV

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

relB

Uniprot No.

P0C079

Research Area

Others

Alternative Names

relB; b1564; JW1556; Antitoxin RelB

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-79aa

Target Protein Sequence

MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

16.1 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

We would like more clarification about the difference between N-terminal or C-terminal Tag?
Please let me know if you have any additional questions.

Very nice to receive your inquiry. As for the difference between N-terminal and C-terminal Tag, the EP protein has already been developed successfully, the tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged
The complete sequence of CSB-EP314742ENV including the tag sequence, any linker sequence and target protein sequence is list as below:

MKETAAAKFERQHMDSPDLGTLVPRGSMADIGSEFHHHHHHHHHHLVPRGSRT

+Target protein sequence:

MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

AAAEQKLISEEDL

The sequence before target protein sequence is N-terminal tag sequence, and after is C-terminal tag sequence.
The detailed explanation for the complete sequence:
S-Tag: KETAAAKFERQHMDS
10xHis: HHHHHHHHHH
Thrombin site: LVPRGS
Linker: RT
Myc-Tag: EQKLISEEDL
This EP protein has been ordered several times by other cutomers, we received the feedback from some customer that he performed the MALDI-TOF identification for this protein, which proves the Mw is correct.The COA of the previous Lot is available upon your request.
The other three proteins are semi-customized proteins that haven't been expressed before, Tag type will be determined during the manufacturing process.
So we can't provide the complete sequence, we can only inform the target protein sequence, and also can't provide further clarification about the tag.
If the customer has desired tag for the YP, BP or MP proteins, you can communicate with us in advance, we can try to express the protein with you desired tag.
If it's failed, we can provide the protein with other tag.

Target Background

Function

Antitoxin component of a type II toxin-antitoxin (TA) system. Counteracts the effect of cognate toxin RelE via direct protein-protein interaction, preventing RelE from entering the ribosome A site and thus inhibiting its endoribonuclease activity. An autorepressor of relBE operon transcription. 2 RelB dimers bind to 2 operator sequences; DNA-binding and repression is stronger when complexed with toxin/corepressor RelE by conditional cooperativity. Increased transcription rate of relBE and activation of relE is consistent with a lower level of RelB in starved cells due to degradation of RelB by protease Lon.; Seems to be a principal mediator of cell death in liquid media.

Protein Families

RelB/DinJ antitoxin family

Database Links

KEGG: ecj:JW1556

STRING: 316385.ECDH10B_1696

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Code	CSB-EP314742ENV
Abbreviation	Recombinant E.coli relB protein
MSDS	MSDS Datasheet
Size	$388
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Escherichia coli Antitoxin RelB (relB)

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