Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

dnaK

Uniprot No.

Q1RGI8

Research Area

Signal Transduction

Alternative Names

dnaK; UTI89_C0016Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70

Species

Escherichia coli (strain UTI89 / UPEC)

Source

E.coli

Expression Region

1-638aa

Target Protein Sequence

MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

73.1 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-638 constitute the expression domain of recombinant Escherichia coli (strain UTI89 / UPEC) dnaK. The expected molecular weight for the dnaK protein is calculated to be 73.1 kDa. Expression of this dnaK protein is conducted in e.coli. Fusion of the N-terminal 6xHis tag into the dnaK encoding gene fragment was conducted, allowing for easier detection and purification of the dnaK protein in subsequent stages.

Escherichia coli chaperone protein DnaK is a key molecular chaperone in bacterial cells, essential for protein folding and quality control. It assists in protein refolding during stress and prevents protein aggregation. In microbiology, DnaK is crucial for bacterial survival under various conditions. In biotechnology, it facilitates recombinant protein expression and production. DnaK's involvement in bacterial virulence makes it a target in infectious disease research. In cell biology, DnaK is a model chaperone for understanding cellular protein folding. Investigating DnaK provides insights into bacterial stress responses, protein homeostasis, and potential applications in biopharmaceuticals, antibiotics, and understanding bacterial pathogenesis.

Code	CSB-EP633459EGW
Abbreviation	Recombinant E.coli dnaK protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Escherichia coli Chaperone protein DnaK (dnaK)

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