Recombinant Escherichia coli Chaperone protein DnaK(dnaK)

In Stock
Code CSB-YP633459EGW
Size US$2959
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details


Recombinant E.coli UTI89 / UPEC dnaK Protein is encoded by dnaK gene — recombinant dnaK DNA — that has integrated foreign plasmids into the Yeast genome, the foreign DNA is replicated along with the host DNA. In addition, the expression of foreign proteins requires the use of specialized expression vectors and often necessitates the significant restructuring of the foreign coding sequence. The subsequent expression process includes small-scale expression, protein identification, large-scale expression, and protein purification. The purity of this recombinant dnaK is 90%+.

DnaK is a protein coding gene that encodes Chaperone protein DnaK. According to some research, DnaK may have the following features.
Stimulation of DnaK's ATPase activity may contribute to its more efficient recycling. ATP-bound DnaK is the predominant form that initiates interactions with substrates for chaperone activity. DnaK also has a prominent function in stabilizing proteins for subsequent folding by GroEL. The DnaK protein contributed to the growth of E. coli by not only protecting some enzymes from denaturation, but also reactivating some enzymes when they misfolded or aggregated. Substrate shuttling between the DnaK and GroEL systems suggests a chaperone network that promotes protein folding.

Purity Greater than 90% as determined by SDS-PAGE.
Target Names dnaK
Uniprot No. Q1RGI8
Alternative Names dnaK; UTI89_C0016Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70
Species Escherichia coli (strain UTI89 / UPEC)
Source Yeast
Expression Region 1-638aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 71.1 kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Acts as a chaperone.
Protein Families Heat shock protein 70 family
Database Links

KEGG: eci:UTI89_C0016

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