Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

rtcB

Uniprot No.

P46850

Research Area

Others

Alternative Names

rtcB; yhgL; b3421; JW3384; RNA-splicing ligase RtcB; EC 6.5.1.8; 3'-phosphate/5'-hydroxy nucleic acid ligase

Species

Escherichia coli (strain K12)

Source

Yeast

Expression Region

1-408aa

Target Protein Sequence

MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQAIETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRDLAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVIYTLRQVVCVKG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

47.2kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Escherichia coli (strain K12) rtcB covers amino acids 1-408. This rtcB protein is theoretically predicted to have a molecular weight of 47.2 kDa. The rtcB protein was expressed in yeast. The rtcB gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant rtcB protein during the following stages.

Escherichia coli RNA-splicing ligase RtcB is an enzyme involved in RNA repair and tRNA splicing. RtcB participates in the repair of damaged or cleaved RNA molecules. RtcB exhibits 3'-phosphate/5'-OH ligase activity, catalyzing the joining of RNA molecules with a 3'-phosphate end to those with a 5'-hydroxyl end. It plays a role in sealing broken RNA ends, facilitating the restoration of functional RNA structures. RtcB is implicated in the splicing of tRNA molecules. In certain organisms, including bacteria, tRNA precursors undergo splicing to remove introns, and RtcB is involved in the ligation of the exons. Understanding the functions of Escherichia coli RNA-splicing ligase RtcB contributes to the broader knowledge of RNA processing, repair mechanisms, and the maintenance of functional RNA molecules within bacterial cells.

Target Background

Function

GTP-dependent RNA ligase that is involved in tRNA splicing and RNA repair. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends. Also acts as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-phosphate (or cyclic-phosphate) and 5'-hydroxy ends that cannot be sealed by classical DNA ligases.

Gene References into Functions

our findings not only provide a physiological function for the RNA ligase RtcB in bacteria but highlight the reversibility of ribosome heterogeneity, a crucial but hitherto undescribed concept for translational regulation. PMID: 27789694
Data show that RtcB seals broken RNAs with 3'-phosphate and 5'-OH ends. PMID: 22474365
RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo PMID: 21757685

Protein Families

RtcB family

Database Links

KEGG: ecj:JW3384

STRING: 316385.ECDH10B_3595

Code	CSB-YP341647ENV
Abbreviation	Recombinant E.coli rtcB protein
MSDS	MSDS Datasheet
Size	$436
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Escherichia coli RNA-splicing ligase RtcB (rtcB)

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